AB9400

Anti-alpha 1 Antitrypsin antibody [G11]

Name: Anti-alpha 1 Antitrypsin antibody [G11]
Brand: Abcam Limited
SKU: AB9400
Rating: 5 (1 reviews)

(1 리뷰)

(16 제품이 사용된 논문 )

Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Full Length Protein corresponding to Human SERPINA1.

2 이미지

AbReview11531****

Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (AB9400)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution

All lanes:

Albumin depleted human patient sera, tissue lysate at 50 µg/mL

Secondary

All lanes:

HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution

Predicted band size: 46 kDa

Observed band size: 52 kDa

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This image is courtesy of an Abreview submitted by Mr John Andersen

CiteAb

Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (AB9400)

alpha 1 Antitrypsin western blot using anti-alpha 1 Antitrypsin antibody [G11] ab9400. Publication image and figure legend from Boylan, K. L., Andersen, J. D., et al., 2010, Proteome Sci, PubMed 20546617.

ab9400 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab9400 please see the product overview.

Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected.

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

G11

Isotype

IgG1

Light chain 타입

unknown

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Full Length Protein corresponding to Human SERPINA1. The exact immunogen used to generate this antibody is proprietary information.

P01009

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR3176514-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

대체 명칭 보기

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

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제품이 사용된 논문 (16)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed37686340

2023

Could the Oxidation of α1-Antitrypsin Prevent the Binding of Human Neutrophil Elastase in COVID-19 Patients?

Applications

Unspecified application

Species

Unspecified reactive species

Maura D'Amato,Monica Campagnoli,Paolo Iadarola,Paola Margherita Bignami,Marco Fumagalli,Laurent Roberto Chiarelli,Giovanni Stelitano,Federica Meloni,Pasquale Linciano,Simona Collina,Giampiero Pietrocola,Valentina Vertui,Anna Aliberti,Tommaso Fossali,Simona Viglio

Current issues in molecular biology 44:2122-2138 PubMed35678672

2022

Investigating the Link between Alpha-1 Antitrypsin and Human Neutrophil Elastase in Bronchoalveolar Lavage Fluid of COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Maura D'Amato,Valentina Vertui,Laura Pandolfi,Sara Bozzini,Tommaso Fossali,Riccardo Colombo,Anna Aliberti,Marco Fumagalli,Paolo Iadarola,Camilla Didò,Simona Viglio,Federica Meloni

Nature genetics 51:1233-1243 PubMed31358993

2019

CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemehsadat Esteghamat,James S Broughton,Emily Smith,Rebecca Cardone,Tarun Tyagi,Mateus Guerra,András Szabó,Nelson Ugwu,Mitra V Mani,Bani Azari,Gerald Kayingo,Sunny Chung,Mohsen Fathzadeh,Ephraim Weiss,Jeffrey Bender,Shrikant Mane,Richard P Lifton,Adebowale Adeniran,Michael H Nathanson,Fred S Gorelick,John Hwa,Miklós Sahin-Tóth,Renata Belfort-DeAguiar,Richard G Kibbey,Arya Mani

PloS one 14:e0214742 PubMed30946770

2019

Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Krutika Patil,Soujanya Yelamanchi,Manish Kumar,Indira Hinduja,T S Keshava Prasad,Harsha Gowda,Srabani Mukherjee

High-throughput 8: PubMed30781848

2019

A Pilot Study to Investigate the Balance between Proteases and α1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients.

Applications

Unspecified application

Species

Unspecified reactive species

Maddalena Cagnone,Davide Piloni,Ilaria Ferrarotti,Monica Di Venere,Simona Viglio,Sara Magni,Anna Bardoni,Roberta Salvini,Marco Fumagalli,Paolo Iadarola,Sabrina Martinello,Federica Meloni

Journal of thrombosis and haemostasis : JTH 14:2023-2026 PubMed27468101

2016

α -antitrypsin Pittsburgh and plasmin-mediated proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

A Henneuse,P Suchon,H Chambost,P E Morange,C Frere,M C Alessi

Journal of translational medicine 13:371 PubMed26608305

2015

Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengguang Guo,Xuejiao Liu,Menglin Li,Chen Shao,Jianling Tao,Wei Sun,Mingxi Li

Biochemical and biophysical research communication 460:596-602 PubMed25800869

2015

Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP.

Applications

Unspecified application

Species

Unspecified reactive species

Hojin Kang,Camille L Duran,Colette A Abbey,Roland R Kaunas,Kayla J Bayless

The Journal of allergy and clinical immunology 133:741-50 PubMed24290289

2013

Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis.

Applications

Species

Human

Peter Valentin Tomazic,Ruth Birner-Gruenberger,Anita Leitner,Britta Obrist,Stefan Spoerk,Doris Lang-Loidolt

Molecular therapy : the journal of the American So 21:954-63 PubMed23459514

2013

A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro.

Applications

Species

Human

Jiamiao Lu,Feijie Zhang,Mark A Kay

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Antitrypsin antibody [G11]

Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (AB9400)

Western blot - Anti-alpha 1 Antitrypsin antibody [G11] (AB9400)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (16)

Product promise