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AB54745

Anti-alpha 1 Fetoprotein antibody [1G7]

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(1 리뷰)

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(4 제품이 사용된 논문 )

Mouse Monoclonal alpha 1 Fetoprotein antibody. Suitable for WB, sELISA, IHC-P, ICC/IF and reacts with Human, Recombinant fragment samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AFP aa 500 to C-terminus.
4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

ab54745 at 10 ug/ml staining alpha Fetoprotein in human HeLa cells by Immunocytochemistry / Immunofluorescence.

This image was generated using the ascites version of the product.

Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)
  • WB

Unknown

Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)

Predicted band size: 68 kDa

false

Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)
  • sELISA

Unknown

Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml

This image was generated using the ascites version of the product.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

1G7

Isotype

IgG

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

sELISA, IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human AFP aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P02771

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/ko/products/unavailable/alpha-1-fetoprotein-antibody-ab8201'>ab8201</a> as Detection.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 13 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.4
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.
Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
See full target information AFP

대체 명칭 보기

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells 12: PubMed36766855

2023

Pharmacological Targeting of the RAGE-NFκB Signalling Axis Impedes Monocyte Activation under Diabetic Conditions through the Repression of SHP-2 Tyrosine Phosphatase Function.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Dorenkamp,Madina Nasiry,Dilvin Semo,Sybille Koch,Ivonne Löffler,Gunter Wolf,Holger Reinecke,Rinesh Godfrey

ACS applied materials & interfaces 13:8823-8831 PubMed33583183

2021

Metal Nanoparticles/MoS Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection.

Applications

Unspecified application

Species

Unspecified reactive species

Engin Er,Ana Sánchez-Iglesias,Alessandro Silvestri,Blanca Arnaiz,Luis M Liz-Marzán,Maurizio Prato,Alejandro Criado

Communications biology 3:636 PubMed33128033

2020

Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Ee Lee,Sun Hee Kim,Dae-Yeul Yu,Eui-Jeon Woo,Myung-Il Kim,Gi-Sang Seong,Sun Min Lee,Jeong-Heon Ko,Yong-Sam Kim

Oncology reports 41:2595-2600 PubMed30816512

2019

Inhibition of apoptosis by miR‑122‑5p in α‑fetoprotein‑producing gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Suguru Maruyama,Shinji Furuya,Kensuke Shiraishi,Hiroki Shimizu,Ryo Saito,Hidenori Akaike,Naohiro Hosomura,Yoshihiko Kawaguchi,Hidetake Amemiya,Hiromichi Kawaida,Makoto Sudo,Shingo Inoue,Hiroshi Kono,Daisuke Ichikawa
제품이 사용된 논문 모두 보기

Product promise

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