Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (AB237969)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab7671)

ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (AB237969)

IHC image of ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7671, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

• WB

Supplier Data

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (AB237969)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 2:

<a href='/ko/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a> at 20 µg

Lane 3:

<a href='/ko/products/unavailable/heart-rabbit-whole-cell-lysate-normal-tissue-ab29072'>ab29072</a> at 20 µg

Lane 4:

<a href='/ko/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a> at 20 µg

Lane 5:

Brain (Human) Membrane Lysate - adult normal tissue at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), at 1/3000 dilution

true

Exposure time: 3min

• WB

Unknown

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (AB237969)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

Lanes 1 - 3:

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969) at 1 µg/mL

Lanes 4 - 6:

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969) at 2 µg/mL

Lanes 7 - 9:

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969) at 5 µg/mL

Lanes 1, 4 and 7:

<a href='/ko/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a> at 20 µg

Lanes 2, 5 and 8:

ab27253 at 20 µg

Lanes 3, 6 and 9:

<a href='/ko/products/unavailable/brain-rat-tissue-lysate-ab7942'>ab7942</a> at 20 µg

Predicted band size: 113 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (AB237969)

Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using ab7671. Band is at 112 kDa.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab7671).

All lanes:

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969) at 1/5000 dilution

All lanes:

Porcine proximal tubule lysate

Predicted band size: 113 kDa

false