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AB323296

Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) (AB323296)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) (AB323296)

Sandwich ELISA of ab323294 with the capture antibody ab323295 dilution at 2 ug/mL and detector antibody ab323296 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) (AB323296)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) (AB323296)

Interpolated concentration of native alpha 2 Macroglobulin was measured at different sample concentrations. Undiluted samples are as follows : human plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) (AB323296)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-alpha 2 Macroglobulin antibody [EPR24425-139] - BSA and Azide free (Detector) (AB323296)

Interpolated concentration of native alpha 2 Macroglobulin was measured at different sample concentrations. Undiluted samples are as follows : human urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24425-139

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB108888

Human alpha 2 Macroglobulin ELISA Kit

Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)

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Sample Prep & Detection Kits

AB270709

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)

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Sample Prep & Detection Kits

AB201808

Gold Conjugation Kit (10 nm, 20 OD)

Conjugation - Gold Conjugation Kit (10 nm, 20 OD) (AB201808)

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  • 0
Primary Antibodies

AB259653

Anti-Factor H antibody [EPR22253-144] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Factor H antibody [EPR22253-144] - BSA and Azide free (Capture) (AB259653)

  • 0
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Primary Antibodies

AB244802

Anti-Apolipoprotein CIII antibody [EPR1371(2)] - BSA and Azide free (Capture)

RabMAb|Recombinant

ELISA - Anti-Apolipoprotein CIII antibody [EPR1371(2)] - BSA and Azide free (Capture) (AB244802)

  • 0
  • 0
Primary Antibodies

AB252636

Anti-GAPDH antibody [EPR20573-71] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-GAPDH antibody [EPR20573-71] - BSA and Azide free (Capture) (AB252636)

  • 0
  • 0
Primary Antibodies

AB228461

Anti-BRAF (mutated V600E) antibody [VE1]

20ul selling size|BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [VE1] (AB228461)

  • 4
  • 7
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab323296 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human alpha 2 Macroglobulin. The recommended pair for sandwich ELISA is:

Capture: ab323295, Human alpha 2 Macroglobulin Capture Antibody (unconjugated)

Detector: ab323296, Human alpha 2 Macroglobulin Detector Antibody (unconjugated)

The reference range value is 0.08-20 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
See full target information A2M

대체 명칭 보기

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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