AB240307

Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal alpha Sarcoglycan antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

ADL, DAG2, SGCA, Alpha-sarcoglycan, Alpha-SG, 50 kDa dystrophin-associated glycoprotein, Adhalin, Dystroglycan-2, 50DAG

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free (AB240307)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free (AB240307)

Immunhistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab189254 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189254).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free (AB240307)

Immunoprecipitation analysis of human skeletal muscle tissue lysate labeling alpha Sarcoglycan using ab189254 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of human skeletal muscle tissue lysate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189254).

All lanes:

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (<a href='/ko/products/primary-antibodies/alpha-sarcoglycan-antibody-epr14773-ab189254'>ab189254</a>)

Predicted band size: 43 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14773

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab240307 is the carrier-free version of ab189254.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Sarcoglycan also known as Adhalin or 50DAG is a protein with a molecular mass of approximately 50 kDa. It is an important component in skeletal and cardiac muscle tissue. This protein is part of the dystrophin-glycoprotein complex (DGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. Alpha Sarcoglycan is primarily expressed in striated muscle cells including those of skeletal and cardiac muscles but also at low levels in other tissues.

Biological function summary

Alpha Sarcoglycan contributes to the integrity and function of muscle fibers by stabilizing the DGC which includes sarcoglycans and other associated proteins. This stabilization provides mechanical support during muscle contraction and stretching. Alpha Sarcoglycan's role in the complex ensures proper signal transduction and protects muscle fibers from injury that may occur through mechanical stress. The protein's interaction with other sarcoglycans such as beta gamma and delta facilitates these structural and signaling functions.

Pathways

Alpha Sarcoglycan is involved in muscle development and maintenance pathways. It plays a role in the mechanotransduction signaling pathway which helps cells convert mechanical stimuli into chemical activity. The DGC including alpha Sarcoglycan works closely with dystrophin an important protein in the process. This relationship is essential for maintaining the structural integrity of muscle fibers and impacts pathways regulating muscle repair and regeneration.

Mutations in the alpha Sarcoglycan gene are linked to limb-girdle muscular dystrophy type 2D (LGMD2D). This condition is characterized by progressive muscle weakness particularly affecting the shoulders and hips. The disruption of alpha Sarcoglycan affects its interaction with other sarcoglycan proteins leading to the disassembly of the DGC and causing increased muscle cell damage. Understanding the role of alpha Sarcoglycan can also provide insights into other disorders related to defects in the DGC.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

See full target information SGCA

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Developmental cell 54:455-470.e5 PubMed32553121

2020

DDX3X Suppresses the Susceptibility of Hindbrain Lineages to Medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deanna M Patmore,Amir Jassim,Erica Nathan,Reuben J Gilbertson,Daniel Tahan,Nadin Hoffmann,Yiai Tong,Kyle S Smith,Thirumala-Devi Kanneganti,Hiromichi Suzuki,Michael D Taylor,Paul Northcott,Richard J Gilbertson

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free (AB240307)

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free (AB240307)

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise