AB155038

Anti-Alpha-synuclein antibody

Name: Anti-Alpha-synuclein antibody
Brand: Abcam Limited
SKU: AB155038
Rating: 4 (3 reviews)

(3 리뷰들)

(4 제품이 사용된 논문 )

Rabbit Polyclonal Alpha-synuclein antibody. Suitable for WB, ICC/IF, IHC-P, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNCA aa 1-150.

대체 명칭 보기

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling alpha Synuclein with ab155038 at 1/250 dilution.

Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody (AB155038)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemical analysis of Frozen sectioned E13.5 Rat brain labelling alpha Synuclein protein expression using ab155038 at a 1/250 dilution.
Blue : DAPI.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for alpha Synuclein protein at cytoplasm and nucleus with ab155038 at a 1/200 dilution (Green). The signal was developed using a goat anti-rabbit IgG antibody.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB155038)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB155038)

Immunofluorescence analysis of DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling alpha Synuclein protein using ab155038 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by an anti-beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for alpha Synuclein protein at cytoplasm and nucleus with ab155038 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, WB, IHC-Fr, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SNCA aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

Due to high sequence homology ab155038 might react with Beta synuclein

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocolusing 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocolusing 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomolecules 11: PubMed34572500

2021

Blood-Derived α-Synuclein Aggregated in the Substantia Nigra of Parabiotic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xizhen Ma,Leilei Chen,Ning Song,Le Qu,Jun Wang,Junxia Xie

Nature communications 12:3101 PubMed34035300

2021

Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Matsui,Junko Ito,Noriko Matsui,Tamayo Uechi,Osamu Onodera,Akiyoshi Kakita

Oncology reports 43:1845-1852 PubMed32236580

2020

NLK interacts with 14‑3‑3ζ to restore the expression of E‑cadherin.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Qingfeng Lin,Tingting Ni,Jiyi Zhao,Feng Lin,Xiangdong Lu,Ye Lv,Shujuan Ren,Zhili Liu,Ting Zhang,Shuyan He,Dong Shen,Weidong Mao

Methods in molecular biology (Clifton, N.J.) 1948:113-121 PubMed30771174

2019

Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Tara A Eastwood,Daniel P Mulvihill

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha-synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB155038)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB155038)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise