AB68206

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal AMPK beta 1 phospho S181 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

3 이미지

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

293T whole cell lysate, membrane incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 20s

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/10000 dilution

Lane 1:

Rat heart lysate at 15 µg

Lane 2:

Rat heart lysate, membrane incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

Lab

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Blocking buffer and dilution concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 15 µg

Lane 2:

Mouse brain lysate, membrane incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2181Y

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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제품 세부 정보

This product has switched from a hybridoma to recombinant production method on 7th February 2025.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes : inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

See full target information PRKAB1 pS181

대체 명칭 보기

AMPK, PRKAB1, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 11:eadw1059 PubMed40864710

2025

genotype influences androgen response in developing murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly N Roeszler,Michael See,Lyra R Meehan,Giscard Lima,Alexander Kolliari-Turner,Sarah E Alexander,Shanie Landen,Harrison D Wood,Chrystal F Tiong,Weiyi Chen,Tomris Mustafa,Peter J Houweling,Nir Eynon,Severine Lamon,Yannis Pitsiladis,David J Handelsman,Fernando J Rossello,Mirana Ramialison,Kathryn N North,Jane T Seto

Journal of cellular and molecular medicine 25:8174-8186 PubMed34309216

2021

LOC100996425 acts as a promoter in prostate cancer by mediating hepatocyte nuclear factor 4A and the AMPK/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuyan Wang,Bin Song,Mingcui Zang,He Ji,He Yang,Shuang Jiang,Xiao Yang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (AB68206)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise