AB9474

Anti-Androgen Receptor antibody [AR 441]

Name: Anti-Androgen Receptor antibody [AR 441]
Brand: Abcam Limited
SKU: AB9474
Rating: 4 (12 reviews)

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Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody detecting Androgen Receptor in Flow Cytometry, IHC-P. Suitable for Human.

- Over 50 publications
- Trusted since 2002

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

ab9474 staining human prostate by IHC-P.

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Flow Cyt

Unknown

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Overlay histogram showing MCF-7 cells stained with ab9474 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Flow Cyt

Unknown

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Overlay histogram showing PC3 cells stained with ab9474 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

AR 441

Isotype

IgG1

Light chain 타입

unknown

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human AR aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P10275

Specificity

This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.

style

left-column