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AB45089

Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2]

5

(3 리뷰들)

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(10 제품이 사용된 논문 )

Mouse Monoclonal Androgen Receptor antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.
2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (AB45089)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (AB45089)

IHC image of Androgen Receptor (phospho S213 + S210) staining in human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45089, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (AB45089)
  • WB

Unknown

Western blot - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (AB45089)

All lanes:

Western blot - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089) at 1 µg/mL

Lane 1:

LNCaP serum starved cells

Lane 2:

LNCaP cells IGF1 incubated for 4h at 0.1 µg/mL

Lane 3:

LNCaP cells incubated with 20 ug LY294002 for 30 min then IGF1 incubated for 4h

Predicted band size: 99 kDa

Observed band size: 110 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

156C135.2

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

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대체품

Primary Antibodies

AB194194

Alexa Fluor® 488 Anti-Androgen Receptor antibody [EPR1535(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Androgen Receptor antibody [EPR1535(2)] (AB194194)

  • 5
  • 3
Primary Antibodies

AB133273

Anti-Androgen Receptor antibody [EPR1535(2)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

  • 5
  • 12
Primary Antibodies

AB108341

Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunohistochemistry - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (AB108341)

  • 5
  • 4
Primary Antibodies

AB52615

Anti-Androgen Receptor antibody [EP670Y]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Androgen Receptor antibody [EP670Y] (AB52615)

  • 4
  • 5
Primary Antibodies

AB227678

Anti-Androgen Receptor antibody [SP242] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP242] - C-terminal (AB227678)

  • 5
  • 2
Primary Antibodies

AB194195

Alexa Fluor® 647 Anti-Androgen Receptor antibody [EPR1535(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Androgen Receptor antibody [EPR1535(2)] (AB194195)

  • 0
  • 0
Primary Antibodies

AB212175

Anti-Androgen Receptor antibody [ER179(2)] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [ER179(2)] - BSA and Azide free (AB212175)

  • 1
  • 1

보완 제품

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB176842

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade

20ul selling size|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (AB176842)

  • 5
  • 15
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p></p>" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. The serine phosphorylation site recognized by ab45089 has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed : 19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.. Isoform 3. Lacks the C-terminal ligand-binding domain and therefore constitutively regulates the transcription of a specific set of canonical AR-target genes, including PSA/KLK3 and TMPRSS2, independently of steroid hormones (PubMed : 19244107, PubMed : 25008967). However, some genes are differentially regulated by full-length AR (isoform 1) and isoform 3. Isoform 3-specific target genes may be regulated independently of FOXA1 expression (PubMed : 25008967).. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
See full target information AR

대체 명칭 보기

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 11:eadw1059 PubMed40864710

2025

genotype influences androgen response in developing murine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly N Roeszler,Michael See,Lyra R Meehan,Giscard Lima,Alexander Kolliari-Turner,Sarah E Alexander,Shanie Landen,Harrison D Wood,Chrystal F Tiong,Weiyi Chen,Tomris Mustafa,Peter J Houweling,Nir Eynon,Severine Lamon,Yannis Pitsiladis,David J Handelsman,Fernando J Rossello,Mirana Ramialison,Kathryn N North,Jane T Seto

Evidence-based complementary and alternative medicine : eCAM 2023:9402155 PubMed37123085

2023

Nourishing Kidney Promoting Ovulation Decoction (NKPOD) Attenuates Polycystic Ovary Syndrome by Downregulating miRNA-224.

Applications

Unspecified application

Species

Unspecified reactive species

Yinhua Guo,Xingli Tong,Peipei Tang,Wenting Zuo,Yong Tan

International journal of molecular sciences 22: PubMed34681618

2021

Androgen Receptor Activation in Glioblastoma Can Be Achieved by Ligand-Independent Signaling through EGFR-A Potential Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Nomi Zalcman,Mijal Gutreiman,Tal Shahar,Michael Weller,Iris Lavon

Mechanisms of development 155:15-26 PubMed30391480

2018

Non-obstructive vas deferens and epididymis loss in cystic fibrosis rats.

Applications

Unspecified application

Species

Unspecified reactive species

Z E Plyler,S E Birket,B D Schultz,J S Hong,S M Rowe,C F Petty,M R Crowley,D K Crossman,T R Schoeb,E J Sorscher

Toxicological sciences : an official journal of the Society of Toxicology 143:126-35 PubMed25324206

2014

Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christina N Robitaille,Patricia Rivest,J Thomas Sanderson

British journal of cancer 104:1452-8 PubMed21468052

2011

Testosterone regulates cell proliferation in aggressive fibromatosis (desmoid tumour).

Applications

Unspecified application

Species

Unspecified reactive species

H Hong,P Nadesan,R Poon,B A Alman

Cancer research 66:10064-72 PubMed17047070

2006

Down-regulation of androgen receptor by 3,3'-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad M R Bhuiyan,Yiwei Li,Sanjeev Banerjee,Fakhara Ahmed,Zhiwei Wang,Shadan Ali,Fazlul H Sarkar

Histopathology 48:787-94 PubMed16722926

2006

Observer variation in immunohistochemical analysis of protein expression, time for a change?

Applications

Unspecified application

Species

Unspecified reactive species

T Kirkegaard,J Edwards,S Tovey,L M McGlynn,S N Krishna,R Mukherjee,L Tam,A F Munro,B Dunne,J M S Bartlett

Journal of neurochemistry 98:355-63 PubMed16638015

2006

A neuroactive steroid 5alpha-androstane-3alpha,17beta-diol regulates androgen receptor level in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Agapova,Paula E Malone,M Rosario Hernandez

The Journal of biological chemistry 278:50902-7 PubMed14555644

2003

Suppression versus induction of androgen receptor functions by the phosphatidylinositol 3-kinase/Akt pathway in prostate cancer LNCaP cells with different passage numbers.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Kuan Lin,Yueh-Chiang Hu,Lin Yang,Saleh Altuwaijri,Yen-Ta Chen,Hong-Yo Kang,Chawnshang Chang
제품이 사용된 논문 모두 보기
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Product promise

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