AB155106

Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)]

Name: Anti-Angiopoietin 2/ANG2 항체 [EPR2891(2)] (ab155106) | Abcam
Brand: Abcam Limited
SKU: AB155106
Rating: 4 (2 reviews)

Anti-Angiopoietin 2/ANG2 항체 [EPR2891(2)]

RabMAb
Recombinant
20ul selling size
각 태그의 뜻

(2 리뷰들)

(49 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Angiopoietin 2/ANG2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 49 publications.

4 이미지

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution

Lane 1:

TF-1 whole cell lysate at 20 µg

Lane 2:

HUVEC whole cell lysate at 20 µg

Lane 3:

ECV-304 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa

false

Unknown

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/1000 dilution

Lane 1:

TF1 cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

ECV-304 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 56 kDa

false

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa

false

CiteAb

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

Angiopoietin 2/ANG2 western blot using anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] ab155106. Publication image and figure legend from Xin, M., He, J., et al., 2018, Pharm Biol, PubMed 31070529.

ab155106 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155106 please see the product overview.

Western blot analysis of rat endometrium tissues. A. WB results for HIF-1α, p-PI3K, p-AKT, Ang1 and Ang2. B-F. The histograms of western blot analysis for HIF-1α (B), p-PI3K (C), p-AKT (D), Ang1 (E) and Ang2 (F) in the five groups. *p< 0.01.

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2891(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.

Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).

See full target information ANGPT2

대체 명칭 보기

Angiopoietin-2, ANG-2, ANGPT2

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제품이 사용된 논문 (49)

Recent publications for all applications. Explore the 전체 목록and refine your search

IBRO neuroscience reports 19:290-299 PubMed40735300

2025

Beclin1-mediated vascular autophagy negatively regulates angiogenesis and secondary neural damage in the thalamus following cerebral cortical infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhi Liu,Yuqian Chen,Xinyan Fan,Jinmin Gu,Shihui Xing

Scientific reports 15:19871 PubMed40473698

2025

Investigating the mechanism of Gentiopicroside in rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Rongyue Jing,Yueyue Chen,Meimei Xu,Xudan Zou,Suling Wu

Materials today. Bio 32:101757 PubMed40290884

2025

ROS-responsive drug delivery system with enhanced anti-angiogenic and anti-inflammatory properties for neovascular age-related macular degeneration therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Zuo,Yining Pan,Yuanli Wang,Wei Wang,Haojie Zhang,Si Zhang,Youru Wu,Jiangfan Chen,Qingqing Yao

Oncology letters 28:604 PubMed39483968

2024

infection downregulates hypoxia‑inducible factor 1α expression to suppress the vascularization and tumorigenesis of liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Runling Wu,Xiao Chen,Huan Chen,Mei Li,Yun Liang

Integrative cancer therapies 23:15347354241273962 PubMed39223822

2024

Chinese Herbal Compound Xiaoliu Pingyi Recipe Inhibits the Growth of Lung Adenocarcinoma by Regulating the Tumor Vascular Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Fei-Ran Yang,Hong-Lin Li,Xi-Wen Hu,Rong Fu,Xiu-Rong Li,Hui-Jie Li

Brain tumor pathology 41:61-72 PubMed38619734

2024

Status of alternative angiogenic pathways in glioblastoma resected under and after bevacizumab treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Taketo Ezaki,Toshihide Tanaka,Ryota Tamura,Kentaro Ohara,Yohei Yamamoto,Jun Takei,Yukina Morimoto,Ryotaro Imai,Yuki Kuranai,Yasuharu Akasaki,Masahiro Toda,Yuichi Murayama,Keisuke Miyake,Hikaru Sasaki

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306248 PubMed38251411

2024

A Nucleic Acid-Based LYTAC Plus Platform to Simultaneously Mediate Disease-Driven Protein Downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Huang,Xujiao Zhou,Yirou Zhang,Miao Xie,Fujun Wang,Jingcan Qin,Han Ye,Hong Zhang,Chuan Zhang,Jiaxu Hong

Annals of gastroenterological surgery 8:163-171 PubMed38250695

2024

Clinical significance of mechanistic target of rapamycin expression in vessels that encapsulate tumor cluster-positive hepatocellular carcinoma patients who have undergone living donor liver transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuya Toshida,Shinji Itoh,Takeo Toshima,Shohei Yoshiya,Ryoichi Goto,Atsuyoshi Mita,Noboru Harada,Kenichi Kohashi,Yoshinao Oda,Tomoharu Yoshizumi

Journal of hepatocellular carcinoma 10:1639-1657 PubMed37791068

2023

A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangjin Huang,Jun Xu,Chunming Xie,Yuejuan Liao,Rong Lin,Yuan Zeng,Fujun Yu

International journal of molecular sciences 24: PubMed37686378

2023

Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Al-Ghadban,Samantha G Walczak,Spencer U Isern,Elizabeth C Martin,Karen L Herbst,Bruce A Bunnell

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)]

Anti-Angiopoietin 2/ANG2 항체 [EPR2891(2)]

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (49)

Product promise