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AB222029

Anti-ANKLE1 antibody

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Rabbit Polyclonal ANKLE1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANKLE1 aa 350 to C-terminus.

대체 명칭 보기

ANKRD41, LEM3, ANKLE1, Structure-specific endonuclease ANKLE1, Ankyrin repeat and LEM domain-containing protein 1, Ankyrin repeat domain-containing protein 41, LEM-domain containing protein 3

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody (AB222029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody (AB222029)

Paraffin embedded human bone marrow stained for ANKLE1 using ab222029 at 1/1000 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ANKLE1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NAG6

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대체품

Primary Antibodies

AB189990

Anti-ANKLE1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

  • 0
  • 0

보완 제품

Primary Antibodies

AB95875

Anti-EYA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA2 antibody (AB95875)

  • 0
  • 0
Primary Antibodies

AB224248

Anti-OXSR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OXSR1 antibody (AB224248)

  • 0
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Primary Antibodies

AB154868

Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)

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Primary Antibodies

AB302883

Anti-ANGPT2 antibody [1F10]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANGPT2 antibody [1F10] (AB302883)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ANKLE1 also known as Ankyrin Repeat and LEM Domain containing 1 or LEM3 is a protein that functions as a DNA endonuclease. This protein has a molecular mass of approximately 63 kDa. It is expressed in various tissues with presence reported in the testis ovaries placenta and to a lesser extent in other tissues. ANKLE1 contains ankyrin repeat domains which often mediate protein-protein interactions. Through its mechanical action it is involved in DNA damage response.
Biological function summary

ANKLE1 acts in the maintenance of genomic stability. It plays a role in resolving DNA structures during replication and repair processes. Evidence suggests ANKLE1 does not function as part of a larger protein complex. Instead it performs its endonuclease activity individually to protect cells from DNA damage. This activity makes it essential for proper cell cycle progression and preventing genomic abnormalities.

Pathways

Functional aspects of ANKLE1 integrate into the DNA damage repair pathway specifically the homologous recombination repair pathway. Its endonuclease action helps in processing DNA intermediates that arise during repair. ANKLE1 works in conjunction with other proteins such as RAD51 which facilitates the strand invasion step of homologous recombination. This collaboration helps ensure genomic integrity is preserved during cell division.

Aberrations in ANKLE1 function or expression have associations with certain cancers such as breast cancer. Its role in safeguarding genomic integrity hints at its protective nature against tumorigenesis. Connections between ANKLE1 and proteins like BRCA1 a well-known key player in DNA repair processes illustrate its potential importance in maintaining genomic stability. Studies continue to explore its precise implications in cancer biology and any potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed : 22399800, PubMed : 27245214, PubMed : 32866453, PubMed : 36825683, PubMed : 37216589). Processes chromatin bridges during cytokinesis by cleaving DNA, both priming TREX1-mediated resolution and directly resolving bridges (PubMed : 36825683). Proper processing of chromatin bridges prevents their breakage during cytokinesis, thereby reducing DNA damage, micronuclei formation, and the activation of immune responses such as the cGAS-STING pathway (PubMed : 36825683).
See full target information ANKLE1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JACC. Basic to translational science 9:792-807 PubMed39070274

2024

Myocardial Infarction Suppresses Protein Synthesis and Causes Decoupling of Transcription and Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Shijie Liu,Vaibhav Deshmukh,Fangfei Wang,Jie Liang,Jenna Cusick,Xiao Li,James F Martin
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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