AB189990

Anti-ANKLE1 antibody - C-terminal

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(2 제품이 사용된 논문 )

Chicken Polyclonal ANKLE1 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANKLE1.

대체 명칭 보기

ANKRD41, LEM3, ANKLE1, Structure-specific endonuclease ANKLE1, Ankyrin repeat and LEM domain-containing protein 1, Ankyrin repeat domain-containing protein 41, LEM-domain containing protein 3

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling ANKLE1 with ab189990 at 5 μg/ml.

Supplier Data

Western blot - Anti-ANKLE1 antibody - C-terminal (AB189990)

Lane 1:

Western blot - Anti-ANKLE1 antibody - C-terminal (ab189990) at 1 µg/mL

Lane 2:

Western blot - Anti-ANKLE1 antibody - C-terminal (ab189990) at 2 µg/mL

All lanes:

293 cell lysate

Predicted band size: 67 kDa

false

주요 정보

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ANKLE1. The exact immunogen used to generate this antibody is proprietary information.

Q8NAG6

Specificity

ab189990 is predicted to not cross-react with ANKLE2.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ANKLE1 also known as Ankyrin Repeat and LEM Domain containing 1 or LEM3 is a protein that functions as a DNA endonuclease. This protein has a molecular mass of approximately 63 kDa. It is expressed in various tissues with presence reported in the testis ovaries placenta and to a lesser extent in other tissues. ANKLE1 contains ankyrin repeat domains which often mediate protein-protein interactions. Through its mechanical action it is involved in DNA damage response.

Biological function summary

ANKLE1 acts in the maintenance of genomic stability. It plays a role in resolving DNA structures during replication and repair processes. Evidence suggests ANKLE1 does not function as part of a larger protein complex. Instead it performs its endonuclease activity individually to protect cells from DNA damage. This activity makes it essential for proper cell cycle progression and preventing genomic abnormalities.

Pathways

Functional aspects of ANKLE1 integrate into the DNA damage repair pathway specifically the homologous recombination repair pathway. Its endonuclease action helps in processing DNA intermediates that arise during repair. ANKLE1 works in conjunction with other proteins such as RAD51 which facilitates the strand invasion step of homologous recombination. This collaboration helps ensure genomic integrity is preserved during cell division.

Aberrations in ANKLE1 function or expression have associations with certain cancers such as breast cancer. Its role in safeguarding genomic integrity hints at its protective nature against tumorigenesis. Connections between ANKLE1 and proteins like BRCA1 a well-known key player in DNA repair processes illustrate its potential importance in maintaining genomic stability. Studies continue to explore its precise implications in cancer biology and any potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed : 22399800, PubMed : 27245214, PubMed : 32866453, PubMed : 36825683, PubMed : 37216589). Processes chromatin bridges during cytokinesis by cleaving DNA, both priming TREX1-mediated resolution and directly resolving bridges (PubMed : 36825683). Proper processing of chromatin bridges prevents their breakage during cytokinesis, thereby reducing DNA damage, micronuclei formation, and the activation of immune responses such as the cGAS-STING pathway (PubMed : 36825683).

See full target information ANKLE1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular neurodegeneration 20:94 PubMed40877951

2025

MS4A6A/Ms4a6d deficiency disrupts neuroprotective microglia functions and promotes inflammation in Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Shan Jiao,Yi-Jun Ge,Liang-Yu Huang,Ying Liu,Bang-Sheng Wu,Piao-Piao Lian,Yi-Ning Hao,Shan-Shan Han,Yi-Ting Li,Kai-Min Wu,Chen-Yun Wu,Tian-Lin Cheng,Peng Yuan,Jin-Tai Yu

International journal of cancer 146:3281-3293 PubMed31509622

2019

ANKLE1 N -Methyladenosine-related variant is associated with colorectal cancer risk by maintaining the genomic stability.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Tian,Pingting Ying,Juntao Ke,Ying Zhu,Yang Yang,Yajie Gong,Danyi Zou,Xiating Peng,Nan Yang,Xiaoyang Wang,Shufang Mei,Yuxing Zhang,Changyi Wang,Rong Zhong,Jiang Chang,Xiaoping Miao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANKLE1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

Western blot - Anti-ANKLE1 antibody - C-terminal (AB189990)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise