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AB122320

Anti-ANKRD23 antibody

4

(2 리뷰들)

|

(1 출판물)

Rabbit Polyclonal ANKRD23 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANKRD23 aa 50-200.

대체 명칭 보기

DARP, ANKRD23, Ankyrin repeat domain-containing protein 23, Diabetes-related ankyrin repeat protein, Muscle ankyrin repeat protein 3

4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)

Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human prostate with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD23 antibody (AB122320)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD23 antibody (AB122320)

Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization : PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)
Western blot - Anti-ANKRD23 antibody (AB122320)
  • WB

Unknown

Western blot - Anti-ANKRD23 antibody (AB122320)

All lanes:

Western blot - Anti-ANKRD23 antibody (ab122320) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Predicted band size: 34 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ANKRD23 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q86SG2

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보완 제품

Primary Antibodies

AB272894

Anti-ANKRD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD1 antibody (AB272894)

  • 0
  • 0
Primary Antibodies

AB249036

Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free (AB249036)

  • 0
  • 0
Primary Antibodies

AB62337

Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y]

RabMAb

Western blot - Anti-Integrin beta 1 (phospho Y783) antibody [EP974(2)Y] (AB62337)

  • 0
  • 0
Primary Antibodies

AB122305

Anti-Leiomodin-3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leiomodin-3 antibody (AB122305)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD23 also known as Ankyrin Repeat Domain-containing protein 23 is a protein that plays a significant role in cellular processes through mechanical interactions. It weighs approximately 34 kDa. ANKRD23 is expressed in various tissues with a notable presence in the brain suggesting its involvement in neurological functions. This target belongs to the ankyrin repeat protein family recognized for specific protein-protein interactions that aid in maintaining cellular structure and signaling.
Biological function summary

ANKRD23 acts as an adaptor protein facilitating interactions between other proteins and influencing signaling pathways. It is known to be part of protein complexes involved in the regulation of cell cycle and apoptotic processes. Through these functions ANKRD23 supports cellular homeostasis and response mechanisms highlighting its importance in maintaining cellular integrity under different physiological conditions.

Pathways

ANKRD23 is associated with the Wnt signaling pathway and the NF-kB signaling pathway. These pathways are important for regulating gene transcription cellular growth and immune responses. ANKRD23 interacts with proteins such as beta-catenin in the Wnt pathway and RELA in the NF-kB pathway which are instrumental in mediating cellular responses to external stimuli and internal regulatory signals.

ANKRD23 has been linked to neurodegenerative diseases and certain cancers. In the context of neurodegenerative diseases ANKRD23's interactions with proteins such as TAU may influence the progression of conditions like Alzheimer's disease. In cancer altered expression of ANKRD23 could affect the regulatory balance within cell signaling pathways potentially interacting with p53 in cell cycle control and apoptosis evasion.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May be involved in the energy metabolism. Could be a molecular link between myofibrillar stretch-induced signaling pathways and muscle gene expression.
See full target information ANKRD23
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of physiology. Cell physiology 313:C327-C339 PubMed28615162

2017

Characterization of muscle ankyrin repeat proteins in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan G Wette,Heather K Smith,Graham D Lamb,Robyn M Murphy
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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