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AB242355

Anti-Annexin-4/ANXA4 antibody [EPR22929-32] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Annexin-4/ANXA4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

ANX4, ANXA4, Annexin A4, 35-beta calcimedin, Annexin IV, Annexin-4, Carbohydrate-binding protein p33/p41, Chromobindin-4, Endonexin I, Lipocortin IV, P32.5, PP4-X, Placental anticoagulant protein II, Protein II, PAP-II

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Sandwich ELISA - Anti-Annexin-4/ANXA4 antibody [EPR22929-32] - BSA and Azide free (Detector) (AB242355)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Annexin-4/ANXA4 antibody [EPR22929-32] - BSA and Azide free (Detector) (AB242355)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272212).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22929-32

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

제품 세부 정보

ab242355 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Annexin A4. The recommended pair for sandwich ELISA is:
Capture: ab242354, Human Annexin A4 Capture Antibody (unconjugated)
Detector: ab242355, Human Annexin A4 Detector Antibody (unconjugated)
The reference range value is 39.0625 - 2500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-4 also known as ANXA4 is a protein with a molecular mass of approximately 36 kDa. It belongs to the annexin family characterized by their ability to bind phospholipids in a calcium-dependent manner. Annexin-4 is widely expressed in various tissues including epithelial cells in the gastrointestinal tract kidney and placenta. This protein associates with the inner surface of the plasma membrane and plays roles in processes like vesicle trafficking and membrane dynamics.
Biological function summary

The protein functions in cellular processes involving membrane-related activities. It does not appear to be part of a larger stable complex but researchers have observed its interaction with cell membranes and involvement in membrane repair and cell signal transduction. Annexin-4 can mediate interactions between the cytoskeleton and the plasma membrane impacting cell motility and structure.

Pathways

Annexin-4 functions in the phospholipid signaling pathways particularly influencing pathways related to calcium signaling. In the calcium-dependent signaling pathway Annexin-4 regulates cellular responses by cooperating with proteins such as calmodulin and other annexins contributing to processes like cell proliferation and apoptosis.

Annexin-4 has shown associations with cancer and inflammatory diseases. Abnormal expression of Annexin-4 has been observed in certain cancer types where it may affect tumor metastasis and progression by modifying cellular adhesion and migration. Additionally its interactions with inflammation-related proteins such as interleukins suggest its involvement in inflammatory responses and disorders like inflammatory bowel disease. Researchers continue to explore its potential as a biomarker for early detection and as a target for therapeutic intervention.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
See full target information ANXA4

Product promise

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