AB198990

Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Annexin-7/ANXA7 antibody. N-terminal. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

ANX7, SNX, OK/SW-cl.95, ANXA7, Annexin A7, Annexin VII, Annexin-7, Synexin

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling ANXA7 with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

ANXA7 was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : U87-MG (human glioblastoma) whole cell extract (Input) 10 μg.
Lane 2 : ab198990 IP in U87-MG (human glioblastoma) whole cell extract.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990)

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

All lanes:

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution

All lanes:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

All lanes:

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

All lanes:

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

All lanes:

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16173

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-7 (ANXA7) also known as synexin is a calcium-dependent phospholipid-binding protein. It has a molecular weight of approximately 51 kDa. This protein is expressed in various tissues with notable expression in the heart and brain. Mechanically ANXA7 plays a role in membrane fusion and exocytosis which are critical processes for cellular communication and transport.

Biological function summary

Annexin-7 functions as an important player in cell proliferation and signal transduction. It forms part of the annexin family of proteins which are known for their role in calcium ion binding and membrane-related activities. Annexin-7 has a role in linking Ca2+ signaling with phospholipid membrane dynamics which suggests its involvement in cellular events like growth and differentiation.

Pathways

Annexin-7 influences both the MAPK and EGFR signaling pathways. These pathways are essential for regulating cell division apoptosis and cellular response to external stimuli. Within these pathways Annexin-7 interacts with proteins such as Ras and PI3K which are important for propagating signals that control cellular behaviors.

Abnormalities in Annexin-7 expression and function associate it with cancer and schizophrenia. In cancer altered ANXA7 function may disrupt normal cell communication and growth pathways contributing to tumorigenesis. In schizophrenia disrupted calcium signaling involving ANXA7 may affect neuronal communication and brain function. In these conditions Annexin-7 interacts with proteins like p53 and NMDA receptors which are critical to the pathology of cancer and neurological disorders respectively.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

See full target information ANXA7

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of gastrointestinal oncology 17:106781 PubMed40837761

2025

regulates cell proliferation, migration, apoptosis and ferroptosis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Zu-Guo Yuan,Kai-Feng Zheng,Ke Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

Immunoprecipitation - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

Western blot - Anti-Annexin-7/ANXA7 antibody [EPR16173] - N-terminal (AB198990)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise