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AB242662

Anti-Annexin V/ANXA5 antibody [EPR20167-5] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Annexin V/ANXA5 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Annexin V/ANXA5 antibody [EPR20167-5] - BSA and Azide free (Capture) (AB242662)
  • sELISA

Unknown

Sandwich ELISA - Anti-Annexin V/ANXA5 antibody [EPR20167-5] - BSA and Azide free (Capture) (AB242662)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab223863).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20167-5

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB14085

Annexin V-FITC Apoptosis Staining / Detection Kit

Functional Studies - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

  • 5
  • 3
Sample Prep & Detection Kits

AB269901

Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®

Schematic Diagram - Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® (AB269901)

  • 0
  • 0
Sample Prep & Detection Kits

AB188215

Gold Conjugation Kit (20 nm, 20 OD)

Conjugation - Gold Conjugation Kit (20 nm, 20 OD) (AB188215)

  • 0
  • 0
Primary Antibodies

AB242931

Anti-MDC antibody [EPR20301-15] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MDC antibody [EPR20301-15] - BSA and Azide free (Detector) (AB242931)

  • 0
  • 0
Primary Antibodies

AB275229

Anti-VILIP1 antibody [EPR23794-168] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-VILIP1 antibody [EPR23794-168] - BSA and Azide free (Detector) (AB275229)

  • 0
  • 0
Primary Antibodies

AB281154

Anti-Chromogranin C/SGII antibody [EPR23876-49] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Chromogranin C/SGII antibody [EPR23876-49] - BSA and Azide free (Capture) (AB281154)

  • 0
  • 0
Primary Antibodies

AB277242

Anti-Sortilin/NT3 antibody [EPR23093-46] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Sortilin/NT3 antibody [EPR23093-46] - BSA and Azide free (Detector) (AB277242)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242662 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Annexin V. The recommended pair for sandwich ELISA is:
Capture: ab242662, Human Annexin V Capture Antibody (unconjugated)
Detector: ab242907, Human Annexin V Detector Antibody (unconjugated)
The reference range value is 781.25 - 25000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Annexin V also known as ANXA5 is a protein with a mass of approximately 35-36 kDa. This protein belongs to the annexin family which includes proteins capable of binding to phospholipids in a calcium-dependent manner. Annexin V is mostly expressed in placental tissue though it can be found in various other tissues like the vascular endothelium. Researchers often utilize annexin V for staining dead cells or identifying apoptosis using assays such as annexin V FITC or 7-AAD staining. It forms part of the annexin V binding buffer which enhances its binding affinity.
Biological function summary

Annexin V has an important role in cell membrane dynamics and signaling. The protein does not function as part of a larger complex but its ability to bind negatively charged phospholipids like phosphatidylserine makes it important in apoptosis. During apoptosis phosphatidylserine translocates to the outer leaflet of the plasma membrane where annexin V marks these apoptotic cells. This property enables annexin V-based assays to detect early stages of apoptosis.

Pathways

Annexin V involvement is seen in pathways associated with blood coagulation and apoptosis. In the apoptosis pathway annexin V interacts with apoptotic markers to identify dying cells. It relates to proteins such as caspases which are key executors of apoptosis. In coagulation annexin V has been shown to interact with prothrombinase complex and can affect calcium ion concentration thereby influencing blood clotting processes.

Annexin V plays a role in antiphospholipid syndrome and cardiovascular diseases. Annexin V has been researched for its connection to antiphospholipid antibodies where it affects normal annexin V function impacting clot formation. In cardiovascular diseases annexin V can interact with proteins such as thrombomodulin influencing thrombotic and atherosclerotic pathways. Understanding these interactions helps researchers develop potential therapeutic approaches for related conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
See full target information ANXA5

대체 명칭 보기

ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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