JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244656

Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free (Capture)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal Antithrombin III/ATIII antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.
2 이미지
Sandwich ELISA - Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free (Capture) (AB244656)
  • sELISA

Lab

Sandwich ELISA - Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free (Capture) (AB244656)

Sandwich ELISA of ab288647 with the capture antibody ab288610 dilution at 2 µg/mL and detector antibody ab244656 dilution at 0.5 µg/mL.

Sandwich ELISA - Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free (Capture) (AB244656)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free (Capture) (AB244656)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab222507).

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Unconjugated

    Anti-Antithrombin III/ATIII antibody [EP5372]

  • Carrier free

    Anti-Antithrombin III/ATIII antibody [EP5372] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5372

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Assay Kits

AB197006

Thrombin Activity Assay Kit (Fluorometric)

Functional Studies - Thrombin Activity Assay Kit (Fluorometric) (AB197006)

  • 0
  • 0
Sample Prep & Detection Kits

AB269899

PE/Texas Red Conjugation Kit - Lightning-Link®

Schematic Diagram - PE/Texas Red Conjugation Kit - Lightning-Link® (AB269899)

  • 0
  • 0
Sample Prep & Detection Kits

AB270703

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (AB270703)

  • 0
  • 0
Primary Antibodies

AB209040

HRP Anti-NDUFV2 antibody [EPR15351(B)]

RabMAb|Recombinant

Western blot - HRP Anti-NDUFV2 antibody [EPR15351(B)] (AB209040)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB189490

Anti-Fibrinogen beta chain antibody [EPR18145-84]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490)

  • 4
  • 1
Assay Kits

AB222507

Human Antithrombin III / ATIII ELISA Kit

Recombinant|SimpleStep

ELISA - Human Antithrombin III / ATIII ELISA Kit (AB222507)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab244656 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Antithrombin III / ATIII. The recommended pair for sandwich ELISA is:
Capture: ab244656, Human Antithrombin III / ATIII Capture Antibody (unconjugated)
Detector: ab244918, Human Antithrombin III / ATIII Detector Antibody (unconjugated)
The reference range value is 0.16-10 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Antithrombin III also known as ATIII or antithrombin 3 is a glycoprotein with a mass of approximately 58 kDa. This protein is mainly synthesized in the liver and circulates in the blood plasma. Antithrombin III functions as a serine protease inhibitor targeting and inhibiting several key enzymes involved in blood coagulation including thrombin and Factor Xa. Its activity is influenced by the presence of heparin which increases its inhibitory effects on these enzymes. This interaction is essential for regulating blood clot formation and maintaining hemostatic balance.
Biological function summary

Antithrombin III plays a critical role in blood clotting by specifically inhibiting clotting factors. It forms complexes with its target enzymes leading to their inactivation and preventing excessive thrombin production. The protein does not form part of larger complexes but acts independently to exert its anticoagulant function. The normal range for antithrombin 3 levels in plasma is important for ensuring a balanced blood coagulation system. Its function helps prevent inappropriate clot formation and ensures smooth blood flow through the circulatory system.

Pathways

Antithrombin III is integral to the coagulation cascade. It is primarily involved in the intrinsic and common coagulation pathways where it regulates thrombin and Factor Xa activity. This regulation prevents the unchecked formation of fibrin clots which can lead to thrombosis. ATIII works closely with proteins like heparin cofactor II and protein C which also modulate the coagulation pathway emphasizing its role in maintaining hemostatic balance. Its function is essential in controlling the coagulation cascade and ensuring that it proceeds appropriately without leading to pathology.

Antithrombin III deficiency can lead to coagulation disorders such as thrombophilia characterized by an increased risk of forming clots. Inherited or acquired deficiencies in antithrombin 3 can result in life-threatening thrombotic events. Furthermore liver diseases which affect the production of ATIII can also result in decreased antithrombin activity compounding coagulation problems. Other proteins related to this process include protein S and plasminogen which also play roles in clot regulation and fibrinolysis. Understanding the connection of antithrombin III to these pathways and diseases is important for developing effective therapeutic strategies.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade (PubMed : 15140129, PubMed : 15853774). AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa (PubMed : 15140129). Its inhibitory activity is greatly enhanced in the presence of heparin.
See full target information SERPINC1

대체 명칭 보기

AT3, PRO0309, SERPINC1, Antithrombin-III, ATIII, Serpin C1
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com