AB36122

Anti-AP-A antibody

Name: Anti-AP-A antibody
Brand: Abcam Limited
SKU: AB36122
Rating: 5 (1 reviews)

(1 리뷰)

(6 제품이 사용된 논문 )

Goat Polyclonal AP-A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ENPEP aa 800-850.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody (AB36122)

IHC-P

AbReview28035****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody (AB36122)

ab36122 staining AP-A in rat kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked using 20% serum for 1 hour at 25°C and then incubated with ab36122 at 3μg/ml for 1 hour. The secondary used was an undiluted HRP conjugated polyclonal.

Image courtesy of Dr Carl Atkinson by Abreview.

Unknown

Western blot - Anti-AP-A antibody (AB36122)

Primary incubated for 1 hour.

All lanes:

Western blot - Anti-AP-A antibody (ab36122) at 1 µg/mL

All lanes:

Human Testis Lysate at 30 µg

Predicted band size: 109 kDa

Observed band size: 170 kDa

true

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ENPEP aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

Q07075

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature, Ando et al. PMID 11994381.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

AP-A also known as Aminopeptidase A is an enzyme that plays a critical role in cellular processes by cleaving N-terminal amino acids from peptide substrates. It has a molecular mass of approximately 100 kDa. AP-A is highly expressed in the brain particularly in the hippocampus and glial cells as well as in other tissues like the kidney and placenta. Its activity has been observed in the synaptic membranes signifying its importance in neurobiological functions.

Biological function summary

Aminopeptidase A participates in the regulation of neuropeptides and peptide hormones. It forms part of the renin-angiotensin system where it influences blood pressure and fluid balance. By converting angiotensin II to angiotensin III AP-A modulates these physiological processes. Moreover it is involved in degrading enkephalins which impacts pain perception and emotional responses. This enzyme interacts with other aminopeptidases in the body contributing to various cellular functions.

Pathways

AP-A significantly impacts the renin-angiotensin system and the endothelin pathway. It influences the cardiovascular system by processing angiotensin peptides working closely with proteins such as angiotensin-converting enzyme (ACE). In the endothelin pathway AP-A affects peptide regulation and is associated with the activity of endothelin-converting enzyme. These pathways demonstrate the enzyme's role in cardiovascular health and homeostasis.

Aminopeptidase A is linked to hypertension and neurological disorders like Alzheimer's disease. Increased activity or expression of AP-A is often seen in the context of high blood pressure where it works in conjunction with ACE to modulate blood pressure levels. In Alzheimer's disease altered AP-A activity affects neuropeptide regulation linking it to beta-amyloid accumulation. These associations highlight the enzyme's potential as a target for therapeutic interventions in hypertension and neurodegenerative diseases.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.

See full target information ENPEP

대체 명칭 보기

CD249, Glutamyl aminopeptidase, EAP, Aminopeptidase A, Differentiation antigen gp160, AP-A, ENPEP

style

left-column

제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of physiology 596:3603-3616 PubMed29863758

2018

Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Qing Han,Lian Xu,Xiao-Feng Tang,Wen-Dong Chen,Yong-Jie Wu,Ping-Jin Gao

International journal of hypertension 2017:3967595 PubMed28421141

2017

Excess of Aminopeptidase A in the Brain Elevates Blood Pressure via the Angiotensin II Type 1 and Bradykinin B2 Receptors without Dipsogenic Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Takuto Nakamura,Masanobu Yamazato,Akio Ishida,Yusuke Ohya

Nature communications 6:10112 PubMed26656251

2015

Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage.

Applications

IHC

Species

Mouse

Efrat Dvash,Michal Har-Tal,Sara Barak,Ofir Meir,Menachem Rubinstein

International journal of molecular medicine 32:795-804 PubMed23942780

2013

High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic β-cells.

Applications

Species

Rat

Carmen Härdtner,Caroline Mörke,Reinhard Walther,Carmen Wolke,Uwe Lendeckel

Hypertension (Dallas, Tex. : 1979) 53:790-7 PubMed19289651

2009

Angiotensin I is largely converted to angiotensin (1-7) and angiotensin (2-10) by isolated rat glomeruli.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Q Velez,Kevin J Ryan,Caroline E Harbeson,Alison M Bland,Milos N Budisavljevic,John M Arthur,Wayne R Fitzgibbon,John R Raymond,Michael G Janech

American journal of physiology. Renal physiology 293:F398-407 PubMed17429035

2007

Characterization of renin-angiotensin system enzyme activities in cultured mouse podocytes.

Applications

Species

Mouse

Juan Carlos Q Velez,Alison M Bland,John M Arthur,John R Raymond,Michael G Janech

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP-A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody (AB36122)

Western blot - Anti-AP-A antibody (AB36122)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise