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AB25482

APC Anti-CD11b antibody [M1/70]

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(17 제품이 사용된 논문 )

Anti-CD11b antibody [M1/70] conjugated to APC - suitable for flow cytometry, and reacts with mouse samples. Clone [M1/70] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - APC Anti-CD11b antibody [M1/70] (AB25482)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD11b antibody [M1/70] (AB25482)

C57Bl/6 bone marrow cells were stained with 0.125μg anti-CD11b (ab25482) (solid line) or 0.125μg APC Rat IgG2b isotype control (dashed line).

관련 conjugated 항체와 다양한 조성의 항체 (6)

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

M1/70

Isotype

IgG2b

Light chain 타입

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information.

P05555

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대체품

Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

  • 4
  • 1
Primary Antibodies

AB187537

Anti-CD11b antibody [EP1345Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - BSA and Azide free (AB187537)

  • 4
  • 1
Primary Antibodies

AB216445

Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (AB216445)

  • 0
  • 0
Primary Antibodies

AB52478

Anti-CD11b antibody [EP1345Y] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (AB52478)

  • 5
  • 5

보완 제품

Assay Kits

AB277412

Human CD11b ELISA Kit

ELISA - Human CD11b ELISA Kit (AB277412)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB303467

Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

  • 0
  • 0
Primary Antibodies

AB313900

Alexa Fluor® 488 Anti-CD11c antibody [KB90]

Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11c antibody [KB90] (AB313900)

  • 0
  • 0
Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

Protect the product from prolonged exposure to light.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.142% Sodium dihydrogen phosphate, 0.1% Gelatin
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze|Store undiluted
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).
See full target information Itgam

대체 명칭 보기

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam
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제품이 사용된 논문 (17)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 26: PubMed40430042

2025

Caveolin-1 Deficiency in Macrophages Alleviates Carbon Tetra-Chloride-Induced Acute Liver Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ruirui Li,Yixue Shu,Yulin Yan,Junyi Zhu,Zilu Cheng,Jie Zhang,Liming Zhu,Yanhua Qiao,Quan Sun

Biology direct 20:61 PubMed40399992

2025

Mechanism of LINC01018/miR-182-5p/Rab27B in the immune escape through PD-L1-mediated CD8 T cell suppression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Su Hu,Guoshuo Chen,Aiping Luo,Hailin Zhao,Dan Li,Biao Peng,Jike Du,Dongdong Luo

Biology methods & protocols 10:bpaf020 PubMed40177524

2025

A generalized protocol for the induction of M2-like macrophages from mouse and rat bone marrow mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ulugbek R Yakhshimurodov,Kizuku Yamashita,Kenji Miki,Takuji Kawamura,Shunsuke Saito,Shigeru Miyagawa

Obesity facts 17:24-36 PubMed37820603

2023

Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqian Li,Xiao Dong,Wenqian He,Huibiao Quan,Kaining Chen,Chaoping Cen,Weiping Wei

Cellular & molecular biology letters 28:79 PubMed37828427

2023

Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huimeng Qi,Zhaoguo Zheng,Qiang Liu

Mediators of inflammation 2022:1108569 PubMed36619207

2022

Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Ping Zhao,Xufeng Ding,Fang Li,Jie Jiang,Hua Huang,Lijiang Ji

EBioMedicine 81:104081 PubMed35660787

2022

Fibrin deposition associates with cartilage degeneration in arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Hügle,Sonia Nasi,Driss Ehirchiou,Patrick Omoumi,Alexander So,Nathalie Busso

Journal of clinical laboratory analysis 36:e24455 PubMed35524480

2022

Dioscin ameliorates inflammatory bowel disease by up-regulating miR-125a-5p to regulate macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Shi,Peichen Zhang,Ruifang Jin,Xiaowei Chen,Lemei Dong,Weichang Chen

PloS one 15:e0228889 PubMed32045450

2020

Mesenchymal stem cells reduce alcoholic hepatitis in mice via suppression of hepatic neutrophil and macrophage infiltration, and of oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Meng Wan,Zhi-Qiang Li,Chang Liu,Yue-Feng He,Men-Jie Wang,Xi-Nan Wu,Yuan Zhang,Yu-Hua Li

Frontiers in immunology 10:2969 PubMed31969881

2020

FA-97, a New Synthetic Caffeic Acid Phenethyl Ester Derivative, Ameliorates DSS-Induced Colitis Against Oxidative Stress by Activating Nrf2/HO-1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Mei,Zihao Wang,Yifan Zhang,Ting Wan,Jincheng Xue,Wei He,Yi Luo,Yijun Xu,Xue Bai,Qi Wang,Yujie Huang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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