JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325381

APC Anti-CD300a antibody [EPR27995-84]

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal CD300a antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.
3 이미지
Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Cells were co-stained with anti human CD14 conjugated to Brilliant Violet 421.

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Cells were co-stained with anti human CD19 conjugated to PE/CY7.

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-CD300a antibody [EPR27995-84] (AB325381)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Negative control : 293T (PMID : 22043923)

관련 conjugated 항체와 다양한 조성의 항체 (5)

  • Unconjugated

    Anti-CD300a antibody [EPR27995-84]

  • Carrier free

    Anti-CD300a antibody [EPR27995-84] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD300a antibody [EPR27995-84]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD300a antibody [EPR27995-84]

  • 578 PE

    PE Anti-CD300a antibody [EPR27995-84]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27995-84

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB209478

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

  • 0
  • 0
Primary Antibodies

AB196159

Alexa Fluor® 647 Anti-Calreticulin antibody [EPR3924] - ER Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB196159)

  • 5
  • 3
Primary Antibodies

AB241332

Anti-TIM 3 antibody [EPR22241]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [EPR22241] (AB241332)

  • 4
  • 1
Primary Antibodies

AB239462

APC Mouse IgG1 kappa [MOPC-21] - Isotype Control

Flow Cytometry - APC Mouse IgG1 kappa [MOPC-21] - Isotype Control (AB239462)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation (PubMed : 10746781, PubMed : 16339535, PubMed : 9701027). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6 (PubMed : 22043923).
See full target information CD300A

대체 명칭 보기

CD300a, CMRF35H, IGSF12, HSPC083, CD300A, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CMRF35-H9, IgSF12, IRp60
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com