AB25348

APC Anti-CD45.1 antibody [A20]

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Anti-CD45.1 antibody [A20] conjugated to APC - suitable for flow cytometry, and reacts with mouse samples. Clone [A20] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - APC Anti-CD45.1 antibody [A20] (AB25348)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD45.1 antibody [A20] (AB25348)

Flow Cytometry analysis of C57Bl/6 (left panel) or SJL (right panel) splenocytes labeling CD45.1 with ab25348 at 0.5 μg (solid line) or Mouse IgG2a (Allophycocyanin) isotype control at 0.5 μg (dashed line).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

A20

Isotype

IgG2a

Light chain 타입

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze|Store undiluted

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD45.1 also known as Leukocyte Common Antigen (LCA) or Protein Tyrosine Phosphatase Receptor Type C (PTPRC) is a protein expressed on the surface of all nucleated hematopoietic cells. It has a molecular mass of approximately 180-220 kDa. This protein acts as a receptor-type protein tyrosine phosphatase. CD45.1 is an essential marker in the immune system particularly found on T cells B cells and other white blood cells. The expression of CD45.1 is seen across leukocytes where it plays a significant role in cell signaling and antigen receptor signaling.

Biological function summary

CD45.1 influences the immune response by modulating signals through antigen receptors. It operates as a single-pass type I membrane protein and is not known to be part of a larger complex. This protein is important in the activation and regulation of T-cells and B-cells by fine-tuning the threshold of signals required for cell activation. The presence of CD45.1 affects multiple processes in adaptive immunity impacting lymphocyte development and their further activation in immune responses.

Pathways

CD45.1 is integral in lymphocyte activation pathways. It influences the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways by dephosphorylating specific kinases like Lck and Fyn which are important in initiating TCR and BCR signaling cascades. Moreover CD45.1 interacts with other proteins such as CD3 and CD19 being essential in the regulation of immune response and maintaining proper signaling amplitudes to ensure effective immune function.

CD45.1 is intricately linked to autoimmune diseases and leukemia. The protein's dysregulation can lead to improper immune signaling contributing to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In leukemia the altered expression of CD45.1 and related proteins such as CD20 often correlates with disease progression and treatment response. Understanding the roles of CD45.1 can facilitate the development of targeted therapies in these contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.

See full target information Ptprc

대체 명칭 보기

CD45, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 8: PubMed36394951

2023

Iron therapy mitigates chronic kidney disease progression by regulating intracellular iron status of kidney macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Edwin Patino,Divya Bhatia,Steven Z Vance,Ada Antypiuk,Rie Uni,Chantalle Campbell,Carlo G Castillo,Shahd Jaouni,Francesca Vinchi,Mary E Choi,Oleh Akchurin

Blood 87:4049-56 PubMed8639760

1996

Enhanced engraftment of hematopoietic progenitor cells in mice treated with granulocyte colony-stimulating factor before low-dose irradiation: implications for gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

M Mardiney,H L Malech

Transplantation 57:249-56 PubMed7906058

1994

The role of host T cell subsets in bone marrow rejection directed to isolated major histocompatibility complex class I versus class II differences of bm1 and bm12 mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

D A Vallera,P A Taylor,J Sprent,B R Blazar

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-CD45.1 antibody [A20]

Flow Cytometry - APC Anti-CD45.1 antibody [A20] (AB25348)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise