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AB278731

APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

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Rabbit Recombinant Monoclonal eIF4EBP1 phospho T37 + T46 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1 pT37 + T46.

대체 명칭 보기

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

1 이미지
Flow Cytometry - APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278731)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278731)

Flow cytometric analysis of Jurkat cells treated with LY294002 and unstained as negative control (blue) or treated with LY294002 and stained (red) or treated with TPA and stained (green) using ab278731.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

4EB1T37T46-A5

Isotype

IgG

Light chain 타입

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human EIF4EBP1 pT37 + T46. The exact immunogen used to generate this antibody is proprietary information.

Q13541

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보완 제품

Primary Antibodies

AB32024

Anti-eIF4EBP1 antibody [Y329]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-eIF4EBP1 antibody [Y329] (AB32024)

  • 0
  • 0
Primary Antibodies

AB195352

Anti-METTL3 antibody [EPR18810]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry - Anti-METTL3 antibody [EPR18810] (AB195352)

  • 5
  • 8
Primary Antibodies

AB7046

Anti-Collagen V antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB7046)

  • 5
  • 11
Primary Antibodies

AB151230

Anti-N6-methyladenosine (m6A) antibody

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (AB151230)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
See full target information EIF4EBP1 pT37 + T46
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of gastrointestinal oncology 16:1622-1634 PubMed40950356

2025

DDX18 promotes growth and metastasis of hepatocellular carcinoma via activating EMT and MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Xu,Xiaoxia Yu,Nannan Zhang,Feiran Wang,Zhong Chen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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