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AB322128

APC Anti-LDL Receptor antibody [EPR1-EUG]

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Rabbit Recombinant Monoclonal LDL Receptor antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human, Mouse samples.
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Flow Cytometry - APC Anti-LDL Receptor antibody [EPR1-EUG] (AB322128)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-LDL Receptor antibody [EPR1-EUG] (AB322128)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Low expression : HEK-293.
Gated on viable cells.

Flow Cytometry - APC Anti-LDL Receptor antibody [EPR1-EUG] (AB322128)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-LDL Receptor antibody [EPR1-EUG] (AB322128)

Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Low expression : LNCaP.
Gated on viable cells.

Flow Cytometry - APC Anti-LDL Receptor antibody [EPR1-EUG] (AB322128)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-LDL Receptor antibody [EPR1-EUG] (AB322128)

Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells.

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Unconjugated

    Anti-LDL Receptor antibody [EPR1-EUG]

  • Carrier free

    Anti-LDL Receptor antibody [EPR1-EUG] - BSA and Azide free

  • 578 PE

    PE Anti-LDL Receptor antibody [EPR1-EUG]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1-EUG

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Mouse, Human, Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

  • 4
  • 10
Primary Antibodies

AB305470

APC Anti-ITK / EMT antibody [Y401]

RabMAb|Recombinant

  • 0
  • 0
Proteins & Peptides

AB316419

Recombinant Human LDLR Protein Standard (His tag)

Sandwich ELISA - Recombinant Human LDLR Protein Standard (His tag) (AB316419)

  • 0
  • 0
Primary Antibodies

AB195020

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)

  • 4
  • 5
Primary Antibodies

AB319077

Alexa Fluor® 488 Anti-PCSK9 antibody [EPR17827-117]

Recombinant|RabMAb

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCSK9 antibody [EPR17827-117] (AB319077)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The LDL Receptor also called LDLR is a protein that plays an important role in the uptake and clearance of low-density lipoproteins (LDL) from the bloodstream. LDLR binds LDL particles facilitating their internalization through receptor-mediated endocytosis. This receptor is expressed mainly in the liver adrenal glands and other tissues involved in cholesterol metabolism. It has an approximate molecular weight of 160 kDa. Researchers often study LDLR using techniques like Western blotting to understand its presence and function.
Biological function summary

The LDL receptor interacts with LDL particles to regulate cholesterol levels in the body. It is part of a cell surface complex that recognizes and binds to apolipoprotein B-100 or apolipoprotein E present on LDL. This interaction initiates internalization of LDL leading to its degradation in lysosomes where cholesterol can be released and used by the cell. Mutations in the gene encoding LDLR can lead to inefficient cholesterol uptake influencing various metabolic processes.

Pathways

LDL receptor activities are integral to lipid metabolism and cholesterol homeostasis. Two important biological pathways that involve LDLR include the cholesterol biosynthesis pathway and the lipoprotein clearance pathway. Within these pathways LDLR collaborates closely with proteins like PCSK9 which modulates its expression and degradation and HMG-CoA reductase an important enzyme in cholesterol synthesis to balance cholesterol levels in the body.

Defects in the LDL receptor are strongly associated with familial hypercholesterolemia and atherosclerosis. These conditions arise from impaired clearance of LDL leading to elevated cholesterol levels which pose risks for cardiovascular diseases. LDLR dysfunctions are linked with the Protein PCSK9 whose gain-of-function mutations can exacerbate hypercholesterolemia by promoting degradation of LDLR while statins aim to increase LDLR expression to lower LDL cholesterol levels.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization.
See full target information Low-density lipoprotein receptor

대체 명칭 보기

Low-density lipoprotein receptor, LDL receptor, Ldlr
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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