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AB314942

APC Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal

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Rabbit Recombinant Monoclonal PDGFR beta antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.
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Flow Cytometry (Intracellular) - APC Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB314942)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB314942)

Flow cytometry overlay histogram showing NIH/3T3 cells stained with ab314942 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314942) (1x 106in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

Isotype control antibody (black line) was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

This antibody gave a positive signal in NIH/3T3 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Unconjugated

    Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal

  • Carrier free

    Anti-PDGFR alpha + PDGFR beta antibody [Y92] - BSA and Azide free

  • Carrier free

    Anti-PDGFR alpha + PDGFR beta antibody [Y92] - Low endotoxin, Azide free

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-PDGFR alpha + PDGFR beta antibody [Y92]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92]

  • HRP

    HRP Anti-PDGFR alpha + PDGFR beta antibody [Y92]

  • 578 PE

    PE Anti-PDGFR alpha + PDGFR beta antibody [Y92] (C-terminal)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y92

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse, Human, Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB252357

Human PDGFRB ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human PDGFRB ELISA Kit (AB252357)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB196376

Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (AB196376)

  • 1
  • 3
Primary Antibodies

AB9361

Anti-beta Galactosidase antibody

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (AB9361)

  • 4
  • 38
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR alpha and PDGFR beta are receptor tyrosine kinases also known as CD140a and CD140b. Both receptors have a molecular mass of about 180 kDa. PDGFR alpha is expressed in a variety of tissues including placenta astrocytes and vascular smooth muscle cells. PDGFR beta is more specific to fibroblasts smooth muscle cells and in the vascular system. These receptors bind platelet-derived growth factors (PDGFs) and become activated through dimerization and autophosphorylation.
Biological function summary

These receptors drive cellular processes like proliferation differentiation and migration. PDGFR alpha and beta operate as a significant part of a receptor complex. They modulate responses in mesenchymal cells and influence developmental pathways. In the vasculature system these receptors play roles in maintaining structure and function of blood vessels. Alterations in receptor activities can affect development of tissues and organs.

Pathways

PDGFR alpha and beta are key players in the PI3K-Akt signaling pathway and MAPK pathway. The activation of these pathways leads to cellular processes like survival and growth. PDGFR activity often interacts with other proteins such as SHP-2 RAS and Akt. These interactions contribute to the regulation of cellular responses to external growth signals embedding PDGFR in a web of intracellular cascade systems.

PDGFRs have linkage to conditions like cancer and fibrotic diseases. Aberrant PDGFR alpha activity has connections to glioblastoma while PDGFR beta alterations often relate to systemic sclerosis. These receptors can work alongside proteins like VEGF and TGF-beta within these diseases. Their dysregulation leads to pathological angiogenesis and abnormal cell proliferation making them targets for therapeutic intervention.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
See full target information PDGFRB

Additional targets

PDGFRA

대체 명칭 보기

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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