AB134406

APC Anti-TRAP/CD40L antibody [24-31]

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Anti-TRAP/CD40L antibody [24-31] conjugated to APC - suitable for flow cytometry, and reacts with human samples. Clone [24-31] is a mouse monoclonal antibody cited in 68 publications.

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Flow Cytometry - APC Anti-TRAP/CD40L antibody [24-31] (AB134406)

Flow Cyt

Unknown

Flow Cytometry - APC Anti-TRAP/CD40L antibody [24-31] (AB134406)

Flow cytometry analysis of unstimulated (left) or stimulated for 6 hours using Cell Stimulation Cocktail (500X) (right) normal Human peripheral blood cells with Anti-Human CD8a PE ab134406 at 5 µL (0.5 µg/test. Cells in the lymphocyte gate were used for analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

24-31

Isotype

IgG1

Light chain 타입

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD40LG. The exact immunogen used to generate this antibody is proprietary information.

P29965

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed : 1280226, PubMed : 31331973). Costimulates T-cell proliferation and cytokine production (PubMed : 8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed : 8617933). Induces the activation of NF-kappa-B (PubMed : 15067037, PubMed : 31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed : 15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

See full target information CD40LG

대체 명칭 보기

CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:18859 PubMed40442395

2025

Platelet aggregation elicits FasL expression and hepatocyte apoptosis in sinusoidal obstruction syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri Higashi,Seiichi Munesue,Masakazu Saeki,Ai Harashima,Kumi Kimura,Yu Oshima,Ryohei Takei,Satoshi Takada,Shinichi Nakanuma,Isamu Makino,Tetsuo Ohta,Shintaro Yagi,Hidehiro Tajima,Yasuhiko Yamamoto

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-TRAP/CD40L antibody [24-31]

Flow Cytometry - APC Anti-TRAP/CD40L antibody [24-31] (AB134406)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise