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AB314250

APC Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Knockout Tested Rabbit Recombinant Monoclonal Vimentin antibody - conjugated to APC. Cytoskeleton marker. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
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Flow Cytometry (Intracellular) - APC Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB314250)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB314250)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and VIM knockout HAP1 stained with ab314250 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314250) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, VIM knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

This antibody gave a positive signal in HAP1 Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

관련 conjugated 항체와 다양한 조성의 항체 (14)

  • Unconjugated

    Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • Chicken version

    Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric)

  • Chicken version

    Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) - BSA and Azide free

  • Chicken version

    Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free

  • Carrier free

    Anti-Vimentin antibody [EPR3776] - BSA and Azide free

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • HRP

    HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 578 PE

    PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3776

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB246526

Human Vimentin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

  • 0
  • 0
Primary Antibodies

AB184675

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)

  • 5
  • 8
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB198367

Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB198367)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/12500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
See full target information VIM

대체 명칭 보기

Vimentin, VIM
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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