AB267464

Anti-Apo-H antibody [EPR23087-228]

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Apo-H antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

대체 명칭 보기

B2G1, APOH, Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Beta-2-glycoprotein I, Apo-H, B2GPI, Beta(2)GPI

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Apo-H with ab267464 at 1/500 dilution (0.94 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma in human hepatocellular carcinoma. The section was incubated with ab267464 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling Apo-H with ab267464 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : HeLa (PMID : 14984368).

Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody [EPR23087-228] (AB267464)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Apo-H with ab267464 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line, and no staining in Hela cells. Negative control : Hela (PMID : 14984368). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-H with ab267464 at 1/500 dilution (0.94 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma in human colon. The section was incubated with ab267464 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Unknown

Immunoprecipitation - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Apo-H was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab267464 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267464 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10ug

Lane 2 : ab267464 IP in HepG2 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab267464 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-Apo-H antibody [EPR23087-228] (ab267464)

Predicted band size: 38 kDa

Observed band size: 50 kDa,60 kDa

false

Lab

Western blot - Anti-Apo-H antibody [EPR23087-228] (AB267464)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot analysis, ab108348 exhibited lower sensitivity compared to ab267464, for which reason we recommend ab108348 as superior alternative.

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1 : 500), and fg-grade ECL substrates are suggested if ab267464 must be used.

Lane 1:

Western blot - Anti-Apo-H antibody [EPR2898(2)] (<a href='/ko/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-Apo-H antibody [EPR23087-228] (ab267464) at 1/1000 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50 kDa,60 kDa

false

Exposure time: 60s

Unknown

Western blot - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 147 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 30894461)

Negative control : Hela (PMID : 14984368).

All lanes:

Western blot - Anti-Apo-H antibody [EPR23087-228] (ab267464) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa,60 kDa

false

Unknown

Western blot - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 180 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 30894461).

All lanes:

Western blot - Anti-Apo-H antibody [EPR23087-228] (ab267464) at 1/1000 dilution

All lanes:

Human plasma at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23087-228

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "In Western blot analysis, <a href='/ko/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a> exhibited lower sensitivity compared to ab267464, for which reason we recommend <a href='/ko/products/primary-antibodies/apo-h-antibody-epr28982-ab108348'>ab108348</a> as superior alternative.Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1:500), and fg-grade ECL substrates are suggested if ab267464 must be used.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein H (Apo-H) also known as β2-glycoprotein I functions mechanically as a plasma protein. It has a molecular weight of approximately 50 kDa and it is mostly expressed in the liver. Apo-H plays a role in the body's immune and coagulation systems binding to anionic surfaces such as phospholipids and preventing blood coagulation. Its ability to interact with negatively charged molecules makes it important in the modulation of the coagulation cascade helping maintain balance between bleeding and clotting.

Biological function summary

Apo-H acts as an anticoagulant and participates in the modulation of immune response. It forms complexes with lipid-bound proteins facilitating the clearance of these complexes from the bloodstream. This interaction is key in processes like platelet aggregation and stabilization of membranes. Apo-H also influences the removal of oxidized proteins and lipids reducing cellular stress and preventing tissue damage.

Pathways

Apo-H plays a critical role in the coagulation cascade and lipid metabolism. It interacts with proteins such as prothrombin and fibrinogen influencing blood coagulation pathways. Apo-H's involvement in the clearance of apoptotic cells integrates into the lipid metabolic pathway highlighting its relationship with apolipoproteins and other lipid-related proteins. These interactions illustrate the multi-functional aspects of Apo-H within these pathways.

Apo-H associates with antiphospholipid syndrome and cardiovascular diseases. These conditions arise from its role in coagulation and immune response where disruptions can lead to increased risk of thrombosis. The protein's interaction with other apolipoproteins such as Apo-A1 highlights possible implications in lipid metabolism disorders which in turn can exacerbate cardiovascular risk. Understanding these associations provides insights into potential therapeutic targets for treating these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

See full target information APOH

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apo-H antibody [EPR23087-228]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Flow Cytometry (Intracellular) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Immunocytochemistry/ Immunofluorescence - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Immunoprecipitation - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Western blot - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Western blot - Anti-Apo-H antibody [EPR23087-228] (AB267464)

Western blot - Anti-Apo-H antibody [EPR23087-228] (AB267464)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Biochemicals

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise