AB249308

Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal APOBEC2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

C->U-editing enzyme APOBEC-2, mRNA(cytosine(6666)) deaminase 2, APOBEC2

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

This data was developed using ab157205, the same antibody clone in a different buffer formulation.

ab157205 showing -ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

This data was developed using ab157205, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue, labeling APOBEC2 using ab157205 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

This data was developed using ab157205, the same antibody clone in a different buffer formulation.

ab157205 showing -ve staining in Human normal uterus.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

This data was developed using ab157205, the same antibody clone in a different buffer formulation.

ab157205 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

This data was developed using ab157205, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-APOBEC2 antibody [EPR11285(B)] (<a href='/ko/products/primary-antibodies/apobec2-antibody-epr11285b-ab157205'>ab157205</a>) at 1/10000 dilution

Lane 1:

Fetal human muscle tissue lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Lane 3:

Mouse muscle tissue lysate at 10 µg

Lane 4:

Fetal human heart tissue lysate at 10 µg

Predicted band size: 26 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11285(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab249308 is the carrier-free version of ab157205.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

APOBEC2 short for Apolipoprotein B mRNA Editing Catalytic Polypeptide-like 2 functionally operates as a C to U RNA-editing enzyme. It belongs to the APOBEC family and has a molecular mass of approximately 26 kDa. Expression of APOBEC2 is mainly in heart and skeletal muscle tissues. Its sequence shares homology with other members of the deaminase family suggesting common ancestral functions.

Biological function summary

APOBEC2 influences muscle differentiation by contributing to RNA editing processes. This protein does not act as part of any known large multiprotein complex but interacts with other cellular components to perform its tasks. By changing specific RNA sequences APOBEC2 can affect the proteins generated in muscle cells influencing muscle fiber formation and maintenance.

Pathways

APOBEC2 plays a role in muscle-related pathways such as myogenesis where it is known to regulate muscle-specific gene expression. The involvement of APOBEC2 in the RNA processing pathway links it with activities concerning the RNA life cycle. It interacts with other members of the APOBEC family such as APOBEC1 which have roles in maintaining genomic integrity and modifying RNA to regulate gene expression.

APOBEC2 shows connection primarily to muscle-related pathologies. Deficiencies or dysfunctions in APOBEC2 can relate to myopathies disorders where muscle fibers do not function properly leading to muscle weakness. The protein is also investigated in the context of muscular dystrophy where its role in muscle maintenance suggests potential implications. In these contexts alterations in APOBEC2 can influence interactions with other muscle-related proteins impacting muscle health and disease outcomes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

See full target information APOBEC2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

Western blot - Anti-APOBEC2 antibody [EPR11285(B)] - BSA and Azide free (AB249308)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise