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AB242804

Anti-Apolipoprotein A II/ApoA-II antibody [EPR2912-55] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Apolipoprotein A II/ApoA-II antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

Apolipoprotein A-II, Apo-AII, ApoA-II, Apolipoprotein A2, APOA2

1 이미지
Sandwich ELISA - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2912-55] - BSA and Azide free (Detector) (AB242804)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Apolipoprotein A II/ApoA-II antibody [EPR2912-55] - BSA and Azide free (Detector) (AB242804)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab184859).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2912-55

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

  • 4
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Primary Antibodies

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Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Anti-B7H4 antibody [EPR20236-9] - BSA and Azide free (AB244746)

  • 0
  • 0
Primary Antibodies

AB272282

Anti-Alcam antibody [EPR22341-182] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Alcam antibody [EPR22341-182] - BSA and Azide free (Detector) (AB272282)

  • 0
  • 0
Primary Antibodies

AB273677

Anti-CD226 antibody [EPR23641-38] - BSA and Azide free (Capture)

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Sandwich ELISA - Anti-CD226 antibody [EPR23641-38] - BSA and Azide free (Capture) (AB273677)

  • 0
  • 0
Primary Antibodies

AB272292

Anti-LDL Receptor antibody [EPR22296-103] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LDL Receptor antibody [EPR22296-103] - BSA and Azide free (Detector) (AB272292)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242804 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Apolipoprotein AII. The recommended pair for sandwich ELISA is:
Capture: ab242556, Human Apolipoprotein AII Capture Antibody (unconjugated)
Detector: ab242804, Human Apolipoprotein AII Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-II (also known as ApoA-II or Apolipoprotein A2) is a pivotal protein component of high-density lipoproteins (HDL) in the plasma. It is encoded by the APOA2 gene in humans and displays a molecular mass of approximately 17.4 kDa. Apolipoprotein A-II is primarily expressed in the liver and secreted into the bloodstream where it plays a direct role in lipid metabolism. Its structural composition includes a highly amphipathic α-helical structure that facilitates interaction with lipids aiding in lipoprotein formation and stability.
Biological function summary

Apolipoprotein A-II serves as a significant regulator of HDL metabolism and lipid transport. It is an integral part of HDL particles contributing to their stability and function. Apolipoprotein A-II influences lecithin-cholesterol acyltransferase (LCAT) activity which affects cholesterol esterification. Although less studied than its counterpart Apolipoprotein A-I ApoA-II's interaction with lipids and proteins such as CETP (cholesteryl ester transfer protein) suggests its involvement in lipid exchange and reverse cholesterol transport processes.

Pathways

Apolipoprotein A-II impacts the reverse cholesterol transport pathway and lipid metabolic processes. ApoA-II works closely with its relative Apolipoprotein A-I both part of the same HDL complex. Together they play essential roles in maintaining cholesterol homeostasis and promoting cholesterol efflux from cells back to the liver. The mechanism by which it modulates the function of lipoprotein lipase and hepatic lipase further implicates its role in lipid metabolism and energy balance processes.

Dysfunction or abnormal levels of Apolipoprotein A-II have associations with cardiovascular diseases and metabolic syndromes. Altered ApoA-II levels correlate with an increased risk of atherosclerosis as its imbalance can drive perturbations in HDL composition and function. Moreover research links Apolipoprotein A-II with insulin resistance linking it with ApoA-I imbalanced lipid transport and metabolism in obesity and diabetes contexts. Understanding these connections may guide therapeutic strategies targeting ApoA-II to mitigate the risks and progression of these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
See full target information APOA2
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Product promise

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