Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] - BSA and Azide free (AB313369)

This data was developed using ab313368, the same antibody clone in a different buffer formulation.

Apolipoprotein A V/APOA5 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab313368 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313368 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab313368 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313368 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] (<a href='/ko/products/primary-antibodies/apolipoprotein-a-v-apoa5-antibody-epr25738-68-ab313368'>ab313368</a>) at 1/1000 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

• WB

Lab

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] - BSA and Azide free (AB313369)

This data was developed using ab313368, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Signal in liver tissue may be weak; increased protein loading, a lower antibody dilution, and a femtogram-level ECL substrate are recommended.

Negative control : skeletal muscle, lung (PMID : 11577099), HeLa, THP-1 (PMID : 21489765).

The 70 kDa band detected in plasma and serum corresponds to albumin.

All lanes:

Western blot - Anti-Apolipoprotein A V/APOA5 antibody [EPR25738-68] (<a href='/ko/products/primary-antibodies/apolipoprotein-a-v-apoa5-antibody-epr25738-68-ab313368'>ab313368</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human serum at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human skeletal muscle tissue lysate at 20 µg

Lane 5:

Human lung tissue lysate at 20 µg

Lane 6:

Human kidney tissue lysate at 20 µg

Lane 7:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 9:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s