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AB246865

Anti-Apolipoprotein O antibody

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Rabbit Polyclonal Apolipoprotein O antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human APOO aa 100-200.

대체 명칭 보기

FAM121B, MIC23, MIC26, My025, UNQ1866/PRO4302, APOO, MICOS complex subunit MIC26, Apolipoprotein O, MICOS complex subunit MIC23, Protein FAM121B

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein O antibody (AB246865)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein O antibody (AB246865)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Apolipoprotein O (green) using ab246865 at 4μg/ml in ICC/IF.

Western blot - Anti-Apolipoprotein O antibody (AB246865)
  • WB

Unknown

Western blot - Anti-Apolipoprotein O antibody (AB246865)

All lanes:

Western blot - Anti-Apolipoprotein O antibody (ab246865) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 22 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human APOO aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BUR5

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보완 제품

Primary Antibodies

AB224565

Anti-MIC19 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MIC19 antibody (AB224565)

  • 0
  • 0
Primary Antibodies

AB110329

Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker

Lab Essentials

Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (AB110329)

  • 5
  • 3
Primary Antibodies

AB122807

Anti-QIL1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-QIL1 antibody (AB122807)

  • 0
  • 0
Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein O (ApoO) also known as mitochondrial apolipoprotein is a protein with a molecular mass of 23 kDa. It interacts with lipoproteins and plays a role in lipid metabolism. ApoO is highly expressed in human tissues including the liver heart and skeletal muscle. It associates with high-density lipoprotein (HDL) particles and has been detected in the mitochondria indicating a potential role in energy metabolism. Researchers have identified this protein as part of the apolipoprotein family which is involved in transporting lipids throughout the body.
Biological function summary

ApoO contributes to the regulation of lipid and energy homeostasis. It is not known to associate with a larger protein complex but it may influence mitochondrial functions due to its localization. ApoO shows potential roles in modulating oxidative stress and maintaining cellular energy through influencing lipid peroxidation and mitochondrial respiration. This suggests that ApoO might play a part in cellular protection against oxidative damage which is important for maintaining the health of cells particularly those with high energy demands like cardiac and muscle cells.

Pathways

ApoO affects the lipid metabolism and energy production pathways. This protein impacts the pathway involved in cholesterol efflux and mitochondrial function. ApoO's action overlaps with other apolipoproteins especially ApoA-I which is a critical component of HDL involved in cholesterol transport. By modulating these pathways ApoO may contribute to systemic lipid balance and influence HDL-related cholesterol pathways an essential process in maintaining cardiovascular health.

ApoO relates to cardiovascular diseases and mitochondrial dysfunction. Abnormalities in ApoO expression or function could contribute to dyslipidemia and heart disease as this protein impacts lipid metabolism and heart tissue energy levels. ApoA-I which partners with ApoO in the HDL complex also plays a significant role in cardiovascular health. Furthermore altered ApoO levels or function could link to mitochondrial disorders affecting mitochondrial energy production and oxidative stress leading to broader implications for diseases involving energy metabolism.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed : 25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed : 24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed : 16956892).
See full target information APOO
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of nanobiotechnology 22:548 PubMed39238028

2024

A nanoscale visual exploration of the pathogenic effects of bacterial extracellular vesicles on host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjae Kang,Min Jeong Kim,Dokyung Jeong,Hyung-Jun Lim,Ga-Eun Go,Uidon Jeong,Eunyoung Moon,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim
제품이 사용된 논문 모두 보기
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Product promise

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