Anti-Aquaporin 1 antibody [EPR11588(B)]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labeling Aquaporin 1 with purified ab168387 at 1/50 dilution (3.9 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with N/A. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Flow Cytometry (Intracellular) analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labelling Aquaporin 1 with purified ab168387 at 1/2000 dilution (0.1 μg/mL, Right) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabelled control - /.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Unglycosylated AQP-1 : 28kDa; Glycosylated AQP-1 : 35-50kDa

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution

Lane 1:

Human heart lysate unboiled at 20 µg

Lane 2:

Human spleen lysate unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 28 kDa

false

• WB

Unknown

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

Unglycosylated AQP-1 : 28kDa; Glycosylated AQP-1 : 35-50kDa

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution

Lane 1:

Mouse kidney lysate boiled at 20 µg

Lane 2:

Mouse kidney lysate unboiled at 20 µg

Lane 3:

Rat kidney lysate boiled at 20 µg

Lane 4:

Rat kidney lysate unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 28 kDa

false

• WB

Lab

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

This blot was produced using 4-20% SDS-PAGE containing 15 μg of mouse heart lysate per lane at 150V for 1hr before being transferred onto a 0.45 μm PVDF membrane at 75V for 1hr. The membrane was then blocked for 1hr using 5% NFDM/TBST, then incubated with ab168387 (1/10,000) at room temperature for 1hr. After being washed three times in TBST, the membrane was incubated with Peroxidase conjugated goat anti-rabbit IgG at 1/2000 dilution for 1hr at room temperature. The membrane was washed three times again. Then the signal was developed using the ECL technique.

ab168387 was stored at a range of temperatures (+4°C, +22°C, +37°C) for 1 week before being tested in WB. The image shows the band intensity remains relatively constant across all storage temperatures, demonstrating that antibody activity is not affected under these conditions. This data was generated as part of a structured antibody stability study supporting Abcam’s transition from cold‑chain to ambient shipping.

All lanes:

Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution

All lanes:

Mouse heart lysate at 15 µg with NDFM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

false

Exposure time: 180s