Anti-ARFGEF2/BIG2 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ARFGEF2/BIG2 antibody (AB236951)

A549 (Human lung carcinoma cell line) cells stained for ARFGEF2/BIG2 using ab236951 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). COunterstained with DAPI (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (AB236951)

Paraffin-embedded human breast cancer tissue stained for ARFGEF2/BIG2 with ab236951 at a 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (AB236951)

Paraffin-embedded human pancreatic cancer tissue stained for ARFGEF2/BIG with ab236951 at a 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.