AB224751

Anti-ARH3 antibody

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Rabbit Polyclonal ARH3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADPRS aa 1-150.

대체 명칭 보기

ADPRHL2, ARH3, ADPRS, ADP-ribosylhydrolase ARH3, ADP-ribose glycohydrolase ARH3, ADP-ribosylhydrolase 3, O-acetyl-ADP-ribose deacetylase ARH3, Poly(ADP-ribose) glycohydrolase ARH3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, [Protein ADP-ribosylserine] hydrolase

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-ARH3 antibody (AB224751)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARH3 antibody (AB224751)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ARH3 (green) using ab224751 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

Paraffin-embedded human Fallopian tube tissue stained for ARH3 using ab224751 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

Paraffin-embedded human testis tissue stained for ARH3 using ab224751 at 1/50 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ADPRS aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NX46

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ARH3 also known as ADP-ribosylhydrolase 3 is an enzyme involved in the removal of ADP-ribose groups from proteins a process known as de-ADP-ribosylation. It has a mass of approximately 39 kDa. The enzyme shows widespread expression in human tissues with higher levels in the brain heart and skeletal muscles. This expression pattern indicates its important role in various cellular processes across different tissue types.

Biological function summary

ARH3 catalyzes the hydrolysis of ADP-ribose units attached to serine residues on target proteins reversing the actions of PARPs (poly ADP-ribose polymerases) especially PARP1. By doing this it plays a role in regulating the post-translational modification of proteins. Although not considered a part of a permanent complex ARH3 often interacts with other protein factors involved in DNA repair and cellular stress responses suggesting its dynamic involvement in cellular regulation.

Pathways

This enzyme functions in DNA repair and cellular stress response pathways. ARH3's activity complements the work of PARP1 by removing ADP-ribose groups added during the DNA damage response. This enzymatic balance is essential to prevent excessive ADP-ribosylation that can affect DNA repair processes. The interaction between ARH3 and PARP1 illustrates the coordinated efforts required to maintain genomic stability and respond to cellular stress.

Mutations or dysregulation of ARH3 are linked to neurodegenerative disorders and cancer. In neurological diseases proteins modified by ARH3-driven de-ADP-ribosylation may alter neuronal survival and function. Similarly in cancer improper ARH3 activity could lead to uncontrolled cell proliferation due to a faulty DNA damage response. Tumor protein 53 (TP53) might interact with ARH3 indicating potential pathways affected in cancerous conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose (PubMed : 21498885, PubMed : 29907568, PubMed : 30045870, PubMed : 30401461, PubMed : 30830864, PubMed : 33186521, PubMed : 33769608, PubMed : 33894202, PubMed : 34019811, PubMed : 34321462, PubMed : 34479984, PubMed : 34625544). Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response (PubMed : 28650317, PubMed : 29234005, PubMed : 30045870, PubMed : 33186521, PubMed : 34019811, PubMed : 34625544). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed : 29480802, PubMed : 33186521, PubMed : 34625544). Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds (PubMed : 16278211, PubMed : 33769608). Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage : free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos (PubMed : 16278211). Also hydrolyzes free poly(ADP-ribose) in mitochondria (PubMed : 22433848). Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins (PubMed : 17075046, PubMed : 21498885). Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers (PubMed : 21498885).

See full target information ADPRS

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in physiology 14:1124444 PubMed36814472

2023

Na and K transport and maturation stage ameloblast modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jake Ngu,Antonius L J J Bronckers,Kaitlin Katsura,Yan Zhang,Pamela K Den Besten

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARH3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ARH3 antibody (AB224751)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARH3 antibody (AB224751)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise