AB153725

Anti-ARL13B antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ARL13B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARL13B aa 100-450.

대체 명칭 보기

ARL2L1, ARL13B, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (AB153725)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (AB153725)

Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling ARL13B using ab153725 at 1/500 dilution.

Supplier Data

Western blot - Anti-ARL13B antibody (AB153725)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ARL13B antibody (ab153725) at 1/10000 dilution

All lanes:

Human plasma at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 48 kDa

false

Unknown

Western blot - Anti-ARL13B antibody (AB153725)

10% SDS PAGE

All lanes:

Western blot - Anti-ARL13B antibody (ab153725) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 49 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ARL13B aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

Q3SXY8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ARL13B also known as ADP-ribosylation factor-like protein 13B is a small GTPase with a molecular mass of approximately 45 kDa. This protein predominantly localizes to the ciliary membrane of cells a specific structure important for cell signaling in eukaryotic organisms. Beyond its association with the cilia ARL13B expression is notable in complex neuronal tissues and renal epithelial cells indicating its diverse roles in various physiological processes.

Biological function summary

ARL13B plays an essential role in the formation and maintenance of primary cilia which are key to cell signaling and sensory processing. ARL13B functions as part of a multiprotein complex within the ciliary membrane essential for the localization and distribution of other ciliary proteins. Through its GTPase activity ARL13B regulates ciliary dynamics and signaling pathways that are fundamental to proper cellular function and development.

Pathways

ARL13B modulates the Sonic Hedgehog (Shh) and Wnt signaling pathways both critical in developmental processes and tissue homeostasis. ARL13B interacts with several proteins in these pathways to control signal transduction such as Gli proteins in the Shh pathway and various cytoplasmic and nuclear factors in the Wnt pathway. ARL13B's role as a signaling regulator highlights its importance in maintaining cellular communication and function.

Mutations or dysregulation of ARL13B are associated with Joubert syndrome and Meckel-Gruber syndrome both developmental disorders affecting the brain and other organs. These conditions arise partly from disrupted cilia function linking ARL13B directly to cilia-related syndromes. In Joubert syndrome ARL13B interacts with proteins such as Cep290 and IFT88 emphasizing its involvement in the pathology of ciliary dysfunctions and providing a focal point for understanding and potentially targeting these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze fit; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development : required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. Plays a role in ciliar trafficking of phosphatidylinositol phosphatase INPP5E in ciliogenesis (PubMed : 38219074). May regulate ARF6- and RAB22A-dependent endocytic recycling traffic (PubMed : 23223633).

See full target information ARL13B

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 15:1418456 PubMed39104395

2024

Pan-cancer analyses reveal the molecular and clinical characteristics of TET family members and suggests that TET3 maybe a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Zhang,Jie Zheng,Jin Liu,Yanxia Li,Guoqiang Xing,Shupeng Zhang,Hekai Chen,Jian Wang,Zhijiang Shao,Yongyuan Li,Zhongmin Jiang,Yingzi Pan,Xiaozhi Liu,Ping Xu,Wenhan Wu

Scientific reports 13:6118 PubMed37059819

2023

Modulation of tau tubulin kinases (TTBK1 and TTBK2) impacts ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Frances M Bashore,Ariana B Marquez,Apirat Chaikuad,Stefanie Howell,Andrea S Dunn,Alvaro A Beltran,Jeffery L Smith,David H Drewry,Adriana S Beltran,Alison D Axtman

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ARL13B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (AB153725)

Western blot - Anti-ARL13B antibody (AB153725)

Western blot - Anti-ARL13B antibody (AB153725)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise