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AB326690

Anti-ARPC2 antibody [SR1558]

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Rabbit Recombinant Monoclonal ARPC2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
5 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [SR1558] (AB326690)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [SR1558] (AB326690)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for ARPC2 using antibody at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [SR1558] (AB326690)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [SR1558] (AB326690)

ICC/IF staining of ARPC2 in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Western blot - Anti-ARPC2 antibody [SR1558] (AB326690)
  • WB

Supplier Data

Western blot - Anti-ARPC2 antibody [SR1558] (AB326690)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 34 kDa
Observed band size : 34 kDa

Exposure time : 4 seconds

All lanes:

Western blot - Anti-ARPC2 antibody [SR1558] (ab326690) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

JK whole cell lysates at 20 µg

Lane 3:

HepG2 whole cell lysates at 20 µg

Lane 4:

MCF-7 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Exposure time: 4s

Western blot - Anti-ARPC2 antibody [SR1558] (AB326690)
  • WB

Supplier Data

Western blot - Anti-ARPC2 antibody [SR1558] (AB326690)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : ARPC2 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-ARPC2 antibody [SR1558] (ab326690) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

ARPC2 knockdown Hela cell lysate at 20 µg

false

Western blot - Anti-ARPC2 antibody [SR1558] (AB326690)
  • WB

Supplier Data

Western blot - Anti-ARPC2 antibody [SR1558] (AB326690)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Mouse liver lysates
Lane 2 : Mouse lung lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 34 kDa
Observed band size : 34 kDa

Exposure time : 7 seconds

All lanes:

Western blot - Anti-ARPC2 antibody [SR1558] (ab326690) at 1/1000 dilution

Lane 1:

Mouse liver lysates at 20 µg

Lane 2:

Mouse lung lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Exposure time: 7s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1558

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB51243

Anti-ARPC5/p16 ARC antibody [EP1551Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

  • 5
  • 5
Primary Antibodies

AB49671

Anti-Arp3 antibody [FMS338]

Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [FMS338] (AB49671)

  • 5
  • 2
Primary Antibodies

AB129018

Anti-Arp2 antibody [EPR7979]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Arp2 antibody [EPR7979] (AB129018)

  • 0
  • 0
Primary Antibodies

AB176842

Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade

20ul selling size|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (AB176842)

  • 5
  • 15
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). Seems to contact the mother actin filament (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC2

대체 명칭 보기

ARC34, PRO2446, ARPC2, Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subunit, p34-ARC
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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