Anti-ARPC5/p16 ARC antibody [EP1551Y]
Anti-ARPC5/p16 ARC 항체 [EP1551Y]
- RabMAb
- Recombinant
- KO Validated
- 20ul selling size
- 각 태그의 뜻
5
(5 리뷰들)
|
(128 제품이 사용된 논문 )
- Clone EP1551Y is the most cited clone to ARPC5/p16 ARC
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 150 publications
ab51243 detects p16 ARC, part of the Arp2/3 complex, which is involved in actin polymerization. ab51243 does not detect the tumor suppressor CDKN2A/p16INK4a involved in cell cycle regulation, which is commonly referred to as p16.
Please note, in some publications using ab51243, it may be described as detecting CDKN2A/p16INK4a; however based on the immunogen and KO validation data, ab51243 only detects p16 ARC.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling ARPC5/p16 ARC with ab51243 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins ab51243 Anti-ARPC5/p16 ARC antibody [EP1551Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IP
Unknown
Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.
Lane 1 (input) : Human fetal brain tissue lysate (10μg)
Lane 2 (+) : ab51243 + human fetal brain tissue lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab51243 in human fetal brain tissue lysate.
For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
- WB
Lab
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Blocking and dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution
Lane 1:
HeLa whole cell lysate at 20 µg
Lane 2:
SKBR-3 whole cell lysate at 20 µg
Lane 3:
MCF-7 whole cell lysate at 20 µg
Lane 4:
HepG2 whole cell lysate at 20 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
- WB
Supplier Data
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution
Lane 1:
Human fetal brain tissue lysate at 10 µg
Lane 2:
Mouse brain tissue lysate at 10 µg
Lane 3:
Rat brain tissue lysate at 10 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
false
- WB
Lab
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : p16 ARC knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab51243 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab51243 was shown to specifically react with p16 ARC when p16 ARC knockout samples were used. Wild-type and p16 ARC knockout samples were subjected to SDS-PAGE. ab51243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)
Predicted band size: 16 kDa
false
- WB
Lab
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
Blocking and dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution
Lane 1:
Human fetal brain tissue lysate at 20 µg
Lane 2:
Mouse brain tissue lysate at 20 µg
Lane 3:
Rat brain tissue lysate at 20 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
관련 conjugated 항체와 다양한 조성의 항체 (8)
-
Anti-ARPC5/p16 ARC antibody [EP1551Y] - BSA and Azide free
-
660 APC
APC Anti-ARPC5/p16 ARC antibody [EP1551Y]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-ARPC5/p16 ARC antibody [EP1551Y]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-ARPC5/p16 ARC antibody [EP1551Y]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-ARPC5/p16 ARC antibody [EP1551Y]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-ARPC5/p16 ARC antibody [EP1551Y]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-ARPC5/p16 ARC antibody [EP1551Y]
-
578 PE
PE Anti-ARPC5/p16 ARC antibody [EP1551Y]
Reactivity 정보
제품 세부 정보
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has been cited over 158 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has been confirmed by testing in knockout samples.
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has 5 independent reviews from customers.
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) specifically detects ARPC5/p16 ARC (UniProt ID: O15511; Molecular weight: 16kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1551Y - ab220800.
Antibody clone EP1551Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, Alexa Fluor® 750 (ab320122, ab320123, ab320124, ab320125, ab320126, ab320127, ab321644).
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The ARPC5 protein is essential for effective actin polymerization making it an important contributor to cell motility and structure. As part of the Arp2/3 complex it interacts with other proteins to stimulate the growth of new actin filaments. This activity is important in processes like endocytosis phagocytosis and cell migration. Furthermore ARPC5 plays a role in maintaining cellular homeostasis and is involved in cellular signaling pathways.
Pathways
Arpc5 influences the regulation of the actin cytoskeleton which intersects with pathways associated with cell movement and morphogenesis. It prominently relates to the WAVE complex working synergistically to regulate actin dynamics. ARPC5's involvement in the Rac1-WAVE-Arp2/3 pathway highlights its relationship with Rac1 a small GTPase known for regulating the cytoskeleton and cellular functions like adhesion and migration.
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
대체 명칭 보기
제품이 사용된 논문 (128)
Recent publications for all applications. Explore the 전체 목록and refine your search
BMC musculoskeletal disorders 26:935 PubMed41063122
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell death discovery 11:449 PubMed41057297
2025
Applications
Unspecified application
Species
Unspecified reactive species
Stem cell research & therapy 16:526 PubMed41024282
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of ginseng research 49:574-584 PubMed40843014
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of ginseng research 49:541-552 PubMed40843012
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in medicine 12:1627412 PubMed40823556
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in immunology 16:1592737 PubMed40799648
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cells 14: PubMed40558480
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cancer & metabolism 13:31 PubMed40533847
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of cellular and molecular medicine 29:e70507 PubMed40387430
2025
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com