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AB51243

Anti-ARPC5/p16 ARC antibody [EP1551Y]

Anti-ARPC5/p16 ARC 항체 [EP1551Y]

5

(5 리뷰들)

|

(128 제품이 사용된 논문 )

Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) is a rabbit monoclonal antibody detecting ARPC5/p16 ARC in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Clone EP1551Y is the most cited clone to ARPC5/p16 ARC
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 150 publications

ab51243 detects p16 ARC, part of the Arp2/3 complex, which is involved in actin polymerization. ab51243 does not detect the tumor suppressor CDKN2A/p16INK4a involved in cell cycle regulation, which is commonly referred to as p16.

Please note, in some publications using ab51243, it may be described as detecting CDKN2A/p16INK4a; however based on the immunogen and KO validation data, ab51243 only detects p16 ARC.
9 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling ARPC5/p16 ARC with ab51243 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins ab51243 Anti-ARPC5/p16 ARC antibody [EP1551Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • IP

Unknown

Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.

Lane 1 (input) : Human fetal brain tissue lysate (10μg)

Lane 2 (+) : ab51243 + human fetal brain tissue lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab51243 in human fetal brain tissue lysate.

For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)

Predicted band size: 16 kDa

Observed band size: 16 kDa

false

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • WB

Lab

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

SKBR-3 whole cell lysate at 20 µg

Lane 3:

MCF-7 whole cell lysate at 20 µg

Lane 4:

HepG2 whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

false

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • WB

Supplier Data

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 16 kDa

false

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • WB

Lab

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : p16 ARC knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab51243 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab51243 was shown to specifically react with p16 ARC when p16 ARC knockout samples were used. Wild-type and p16 ARC knockout samples were subjected to SDS-PAGE. ab51243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)

Predicted band size: 16 kDa

false

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)
  • WB

Lab

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (AB51243)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Carrier free

    Anti-ARPC5/p16 ARC antibody [EP1551Y] - BSA and Azide free

  • 660 APC

    APC Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ARPC5/p16 ARC antibody [EP1551Y]

  • 578 PE

    PE Anti-ARPC5/p16 ARC antibody [EP1551Y]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1551Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is specific to the p16 ARC protein, database link: <a href="http://www.uniprot.org/uniprot/O15511" tabindex="-1">O15511</a>. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.</p><p>Please note p16 ARC is a component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization, while CDKN2A/p16INK4a is a tumor suppressor involves in cell cycle regulation. For CDKN2A/p16INK4a antibody, please use ab108349, ab211542&#46;</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/70", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

제품 세부 정보

Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.

Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has been cited over 158 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has been confirmed by testing in knockout samples.

Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) has 5 independent reviews from customers.

Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) specifically detects ARPC5/p16 ARC (UniProt ID: O15511; Molecular weight: 16kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EP1551Y - ab220800.

Antibody clone EP1551Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, Alexa Fluor® 750 (ab320122, ab320123, ab320124, ab320125, ab320126, ab320127, ab321644).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ARPC5 also known as p16 ARC is a protein with a molecular weight of roughly 16 kilodaltons. It localizes largely in the cytoplasm and expresses in many cell types across species. The ARPC5 protein is a subunit of the Arp2/3 complex a vital component responsible for actin nucleation and branching during cytoskeleton remodeling. Researchers often examine ARPC5 through techniques such as western blot and immunohistochemistry using specific antibodies like anti-p16 or ARPC5 anticuerpo to identify its presence and distribution.
Biological function summary

The ARPC5 protein is essential for effective actin polymerization making it an important contributor to cell motility and structure. As part of the Arp2/3 complex it interacts with other proteins to stimulate the growth of new actin filaments. This activity is important in processes like endocytosis phagocytosis and cell migration. Furthermore ARPC5 plays a role in maintaining cellular homeostasis and is involved in cellular signaling pathways.

Pathways

Arpc5 influences the regulation of the actin cytoskeleton which intersects with pathways associated with cell movement and morphogenesis. It prominently relates to the WAVE complex working synergistically to regulate actin dynamics. ARPC5's involvement in the Rac1-WAVE-Arp2/3 pathway highlights its relationship with Rac1 a small GTPase known for regulating the cytoskeleton and cellular functions like adhesion and migration.

Imbalances in ARPC5 function can link to cancer progression and neurodegenerative diseases. Aberrant activity of the Arp2/3 complex which involves ARPC5 associates with increased cell invasion and metastasis in cancer. Additionally disruptions in ARPC5 activity connect to neurodegenerative conditions where cytoskeletal arrangement alterations play a role. The protein's interaction with others in the complex such as Rac1 further deepens its involvement in these diseases signaling potential targets for therapeutic intervention.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC5

대체 명칭 보기

ARC16, ARPC5, Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC

제품이 사용된 논문 (128)

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제품이 사용된 논문 모두 보기

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