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AB326344

Anti-ARRDC1 antibody [EPR30212-549] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal ARRDC1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-ARRDC1 antibody [EPR30212-549] - BSA and Azide free (Detector) (AB326344)
  • sELISA

Lab

Sandwich ELISA - Anti-ARRDC1 antibody [EPR30212-549] - BSA and Azide free (Detector) (AB326344)

Sandwich ELISA with the capture antibody ab326343 dilution at 2 µg/mL and detector antibody ab326344 dilution at 0.4 µg/mL after biotin conjugation.

Sandwich ELISA - Anti-ARRDC1 antibody [EPR30212-549] - BSA and Azide free (Detector) (AB326344)
  • sELISA

Lab

Sandwich ELISA - Anti-ARRDC1 antibody [EPR30212-549] - BSA and Azide free (Detector) (AB326344)

Interpolated concentration of native ARRDC1 was measured at different sample concentrations. Undiluted samples are as follows : T-47D cell extract (125 µg/mL) and HEK-293 cell extract (250 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30212-549

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

제품 세부 정보

ab326344 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ARRDC1. The recommended pair for sandwich ELISA is:

Capture: ab326343, Human ARRDC1 Capture Antibody (unconjugated)

Detector: ab326344, Human ARRDC1 Detector Antibody (unconjugated)

The reference range value is 39.1-2500 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates (PubMed : 23886940, PubMed : 27462458). Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles (PubMed : 27462458). More generally, plays a role in the extracellular transport of proteins between cells through the release in the extracellular space of microvesicles (PubMed : 22315426). By participating in the ITCH-mediated ubiquitination and subsequent degradation of NOTCH1, negatively regulates the NOTCH signaling pathway (PubMed : 23886940).
See full target information Arrestin domain-containing protein 1

대체 명칭 보기

Arrestin domain-containing protein 1, Alpha-arrestin 1, ARRDC1

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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