AB309492

Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Aryl hydrocarbon Receptor antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse samples.

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Aryl hydrocarbon receptor, Ah receptor, AhR, Ahr

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mice breast carcinoma labelling Aryl hydrocarbon Receptor with ab309491 at a concentration of 1/200 dilution. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin.

Positive staining on mouse breast carcinoma.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mice liver labelling Aryl hydrocarbon Receptor with ab309491 at a concentration of 1/200 dilution. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin.

Positive staining on mouse liver.

Flow Cytometry (Intracellular) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast, Left) / HEPA1-6 (mouse hepatoma epithelial cell, Right) cells labelling Aryl hydrocarbon Receptor with ab309491 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Neuro-2a(PMID : 16956419).

Lab

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Ahr-KO homozygous mice (Strain ID : T012419).

All lanes:

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] (<a href='/ko/products/primary-antibodies/aryl-hydrocarbon-receptor-antibody-epr27537-70-ab309491'>ab309491</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse lung tissue lysate (male) at 20 µg

Lane 2:

Ahr knockout mouse lung tissue lysate (male case1) at 20 µg

Lane 3:

Ahr knockout mouse lung tissue lysate (male case2) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Neuro-2a(PMID : 16956419) Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Lane 1:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 2:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Pancreas In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse pancreas tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

This data was developed using ab309491, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mice lung tissue labelling Aryl hydrocarbon Receptor with ab309491 at a concentration of 1/200 dilution. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The primary antibody Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin.

Positive staining on (A) Lung tissue from wild-type C57BL/6J mice, no staining on (B) Lung tissue from Ahr knockout mice. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Ahr-KO homozygous mice (Strain ID : T012419).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27537-70

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.

Biological function summary

The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.

Pathways

The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.

Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed : 10639156, PubMed : 10973493, PubMed : 1314586, PubMed : 33436590, PubMed : 7961644, PubMed : 7969080, PubMed : 8806883, PubMed : 9427285). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (By similarity). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed : 20106950, PubMed : 28396409). Xenobiotics can act as ligands : upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed : 10639156, PubMed : 10973493, PubMed : 1314586, PubMed : 7961644, PubMed : 7969080, PubMed : 8806883, PubMed : 9427285). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (By similarity). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (By similarity). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (By similarity). Acts as a negative regulator of anti-tumor immunity : indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (By similarity). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (By similarity). Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed : 20106950). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28396409).

See full target information Ahr

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Flow Cytometry (Intracellular) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Western blot - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aryl hydrocarbon Receptor antibody [EPR27537-70] - BSA and Azide free (AB309492)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise