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AB238726

Anti-ASAHL/PLT antibody

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Rabbit Polyclonal ASAHL/PLT antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAAA aa 1-200.

대체 명칭 보기

ASAHL, PLT, NAAA, N-acylethanolamine-hydrolyzing acid amidase, Acid ceramidase-like protein, Acylsphingosine deacylase NAAA, N-acylsphingosine amidohydrolase-like, ASAH-like protein

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAHL/PLT antibody (AB238726)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAHL/PLT antibody (AB238726)

Paraffin-embedded human liver tissue stained for ASAHL/PLT using ab238726 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NAAA aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q02083

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보완 제품

Primary Antibodies

AB154193

Anti-Von Willebrand Factor antibody [EPSISR15]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

  • 0
  • 0
Primary Antibodies

AB272862

Anti-TRPV2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV2 antibody (AB272862)

  • 0
  • 0
Primary Antibodies

AB234832

Anti-ZMPSTE24 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (AB234832)

  • 0
  • 0
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity greater than 95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ASAHL also known as N-acylsphingosine amidohydrolase or PLT is an enzyme that catalyzes the hydrolysis of ceramide into sphingosine and a free fatty acid. The molecular mass of ASAHL is approximately 45 kDa. This enzyme is highly expressed in many tissues including the brain liver kidney and skeletal muscles. Its activity is essential for the metabolism of sphingolipids playing an important role in maintaining cellular lipid balance and signaling.
Biological function summary

ASAHL participates in regulating apoptosis and cell differentiation by controlling levels of bioactive sphingolipids. Although ASAHL operates as a single enzyme and not part of a larger protein complex its action significantly influences intracellular processes by modulating ceramide levels. Ceramide serves as a precursor for signaling molecules and its degradation by ASAHL leads to the generation of sphingosine which is a lipid mediator involved in various cellular responses including those mediated by interleukin pathways.

Pathways

ASAHL plays a vital part in the sphingolipid metabolism pathway. This pathway influences numerous cellular processes related to cell growth differentiation and apoptosis. The enzyme's activity is closely related to other proteins such as ceramide synthases which are involved in ceramide biosynthesis and sphingosine kinase which phosphorylates sphingosine to produce sphingosine-1-phosphate. By interacting with these proteins ASAHL helps maintain the balance of sphingolipid metabolites that control cellular homeostasis.

ASAHL has significant connections to neurodegenerative diseases and metabolic disorders. Alterations in the enzyme's function or expression can lead to an imbalance in ceramide and sphingosine levels contributing to pathologies such as Alzheimer's disease and insulin resistance. The enzyme ASAHL associates with proteins involved in inflammatory responses potentially linked through pathways involving interleukin-1β (IL-1β) and its inhibitors such as anti-PLT and diacerein which further underlines its significance in disease mechanisms related to inflammation and metabolic regulation.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Degrades bioactive fatty acid amides to their corresponding acids, with the following preference : N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine (PubMed : 15655246, PubMed : 17980170, PubMed : 18793752, PubMed : 22825852, PubMed : 30301806). Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine (PubMed : 15655246).
See full target information NAAA
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 43:208 PubMed39061061

2024

SLC14A1 and TGF-β signaling: a feedback loop driving EMT and colorectal cancer metachronous liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Zhang,Yumeng Yang,Xuan Qi,Peng Cui,Yi Kang,Haiyi Liu,Zhigang Wei,Haibo Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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