AB236996

Anti-ASC1 antibody

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Rabbit Polyclonal ASC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIP4 aa 350-550.

대체 명칭 보기

RQT4, TRIP4, Activating signal cointegrator 1, ASC-1, Thyroid receptor-interacting protein 4, TR-interacting protein 4, TRIP-4

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (AB236996)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (AB236996)

Paraffin-embedded human lung tissue stained for ASC1 using ab236996 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ASC1 antibody (AB236996)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASC1 antibody (AB236996)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASC1 (green) using ab236996 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-ASC1 antibody (AB236996)

All lanes:

Western blot - Anti-ASC1 antibody (ab236996) at 1/500 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 66 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TRIP4 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

Q15650

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity > 95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ASC1 protein also known as ASC-1 is a multifunctional protein involved in several cellular processes. It has a mass of approximately 41 kDa and is primarily expressed in the nucleus of cells. Some studies highlight its presence in diverse tissues indicating a potential broad involvement in different cellular activities. While the protein itself is known common names like ASCONE procedure refer to specialized contexts or nomenclature systems related to ASC1.

Biological function summary

ASC1 plays a significant role in the regulation of transcription and acts as a coactivator of nuclear receptors. It forms part of a larger transcription regulatory complex partnering with other coactivators and transcriptional machinery to enhance gene expression. Its interaction capacity signifies its importance in modulating gene expression patterns thereby affecting cellular physiology at the transcriptional regulation level.

Pathways

ASC1 engages primarily with the nuclear receptor signaling pathways and transcription regulation pathways. Within these pathways it interacts with other proteins like steroid receptor coactivators to modulate various responses to hormonal signals. This integration into complex pathways highlights ASC1's importance in maintaining cellular homeostasis and response to external signals.

ASC1 is linked to cancers particularly hormone-responsive cancers like breast and prostate cancer. Its interaction with nuclear receptors like estrogen receptor and androgen receptor connects ASC1 to the regulation of these cancers' progression. Moreover mutations or dysregulation of ASC1 can contribute to the aberrant expression of genes that promote tumorigenicity. Understanding ASC1 interactions with other coactivators can provide insight into potential therapeutic targets for these cancers.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription (PubMed : 10454579, PubMed : 25219498). May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions (PubMed : 10454579, PubMed : 25219498). Plays a role in thyroid hormone receptor and estrogen receptor transactivation (PubMed : 10454579, PubMed : 25219498). Also involved in androgen receptor transactivation (By similarity). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1 (PubMed : 12077347). Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B (PubMed : 12077347). May play a role in the development of neuromuscular junction (PubMed : 26924529). May play a role in late myogenic differentiation (By similarity). Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 32099016, PubMed : 32579943, PubMed : 36302773).

See full target information TRIP4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 9:eadj4198 PubMed37862421

2023

Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoyuki Shiota,Zhucui Li,Guan-Yuan Chen,Kevin L McKnight,Takayoshi Shirasaki,Bryan Yonish,Heyjeong Kim,Ethan J Fritch,Timothy P Sheahan,Masamichi Muramatsu,Maryna Kapustina,Craig E Cameron,You Li,Qibin Zhang,Stanley M Lemon

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (AB236996)

Immunocytochemistry/ Immunofluorescence - Anti-ASC1 antibody (AB236996)

Western blot - Anti-ASC1 antibody (AB236996)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise