AB276071

Anti-ASF1b antibody [004]

Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal ASF1B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus.

대체 명칭 보기

Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, hAsf1, hAsf1b, CIA-II, hCIA-II, ASF1B

2 이미지

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Immunoprecipitation - Anti-ASF1b antibody [004] (AB276071)

ASF1b was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate using 0.5 μL ab276071 and 60 μg of Immunomagnetic beads Protein G. Western blot was performed from the immunoprecipitate using ab276071 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.

Performed under reducing conditions.

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Immunoprecipitation - Anti-ASF1b antibody [004] (ab276071)

Predicted band size: 22 kDa

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Western blot - Anti-ASF1b antibody [004] (AB276071)

All lanes:

Western blot - Anti-ASF1b antibody [004] (ab276071) at 1/500 dilution

All lanes:

K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

Secondary

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Rabbit IgG Dylight 800-labeled antibody (H+L) at 1/5000 dilution

Predicted band size: 22 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

004

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human ASF1B aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVP2

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.5-2 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ASF1b also known as Anti-Silencing Function 1B is a histone chaperone that weighs approximately 22 kDa. This protein is part of the histone fold family facilitating the transfer of histone H3/H4 dimers either into histone octamers or back to the nucleosome. ASF1b is expressed in various human tissues with significant expression in proliferating cells. Its unique ability to influence chromatin assembly positions it as an important player within cellular dynamics.

Biological function summary

The protein assists in the maintenance and regulation of chromatin structure impacting DNA replication and transcription. ASF1b interacts with histone H3/H4 complexes and often works in coordination with CAF-1 forming a multi-protein complex important for nucleosome assembly during DNA replication. Through these interactions ASF1b plays a role in both maintaining genomic stability and facilitating proper cell cycle progression.

Pathways

ASF1b contributes to the DNA replication and repair pathways required for accurate duplication of genetic material. In the DNA damage response ASF1b offers histone supply critical for chromatin structure restoration post-repair. It also interacts with proteins like ATR and CHK1 aiding in the checkpoint response to DNA damage to prevent genomic instability. However its precise regulation within these pathways can have significant cellular consequences related to genome integrity.

The aberrant expression of ASF1b has links to cancer progression most notably in breast and prostate cancers. Overexpression can drive unchecked cell proliferation correlating with aggressive cancer phenotypes. ASF1b's association with oncogenic pathways involves partner proteins such as p53 where ASF1b indirectly influences its tumor suppressor pathway through modulation of chromatin dynamics showcasing its fundamental role in both normal and pathological cell processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251, PubMed : 21454524, PubMed : 26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) : specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed : 20953179, PubMed : 21454524, PubMed : 26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Required for gonad development (PubMed : 12842904).

See full target information ASF1B

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 23:105 PubMed39844281

2025

Knockout IL4I1 affects macrophages to improve poor efficacy of CD19 CAR-T combined with PD-1 inhibitor in relapsed/refractory diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Yi Zhang,Hairong Xiao,Qingxi Liu,Mingfeng Zhao

World journal of gastrointestinal oncology 14:2353-2366 PubMed36568946

2022

Anti-silencing function 1B knockdown suppresses the malignant phenotype of colorectal cancer by inactivating the phosphatidylinositol 3-kinase/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gen-Hua Yu,Xu-Feng Gong,Ying-Ying Peng,Jun Qian

Neoplasma 69:361-369 PubMed35103478

2022

ASF1B enhances migration and invasion of lung cancers cell via regulating the P53-mediated epithelial-mesenchymal transformation (EMT) signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Lina Xiao,Deng Pan,Lingzhi Hu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASF1b antibody [004]

Immunoprecipitation - Anti-ASF1b antibody [004] (AB276071)

Western blot - Anti-ASF1b antibody [004] (AB276071)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise