Anti-ASFV p54 antibody [GT1075] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

ab327555 detects ASFV p54 protein by immunofluorescent analysis.
Sample : Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : ASFV p54 stained by ab327555 diluted at 1 : 1000.
Blue : Fluoroshield with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

ab327555 detects ASFV p54 protein at cytoplasm by immunohistochemical analysis.
Sample : African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block.
Green : ASFV p54 stained by ab327555 diluted at 1 : 1000.
Blue : Fluoroshield with DAPI.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• sELISA

Supplier Data

Sandwich ELISA - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture : ab327555 (5 μg/mL)
Detection : HRP-conjugated ASFV p54 antibody (1 μg/mL)

• sELISA

Supplier Data

Sandwich ELISA - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture : ab327555 (5 μg/mL)
Detection : ASFV p54 antibody (1 μg/mL)

• WB

Supplier Data

Western blot - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ab327555 diluted at 1 : 5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (ab327555) at 1/5000 dilution

Lane 1:

DDDDK-tag tagged non-transfected 293T whole cell extract at 30 µg

Lane 2:

DDDDK-tag tagged ASFV p54 transfected 293T whole cell extract at 30 µg

false

• LFA

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Detection of ASFV p54 (ECD) protein, His Tag by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody
Detection : ab327555

• LFA

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Detection of ASFV p54 (ECD) protein, His Tag (0.2 μg/ml) by lateral flow assay.
Capture : Biotin-labeled ab327555 (3.32 μg/ml).
Detection : FITC-labeled ASFV p54 antibody (3.32 μg/ml).

• LFA

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture : ab327555
Detection : ASFV p54 antibody

Samples (40 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

• LFA

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Detection of ASFV p54 (ECD) protein, His Tag by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody
Detection : ab327555

• LFA

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture : ab327555
Detection : ASFV p54 antibody

Samples (40 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

• LFA

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [GT1075] - BSA and Azide free (AB327555)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody
Detection : ab327555

Samples (80 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer