AB216521

Anti-ASGR2 antibody [EPR16975] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ASGR2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

CLEC4H2, ASGR2, Asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

This IHC data was generated using the same anti-Asiaglycoprotein Receptor 2 antibody clone, EPR16975, in a different buffer formulation (cat# ab200196).

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ASGR2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm and cell membrane staining on Human liver tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ASGR2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

No staining on Human skeletal muscle tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200196).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

ASGR2 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab200196 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab200196 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal liver whole cell lysate 10 μg (Input).

Lane 2 : ab200196 IP in Human fetal liver whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200196 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200196).

All lanes:

Immunoprecipitation - Anti-ASGR2 antibody [EPR16975] (<a href='/ko/products/primary-antibodies/asgr2-antibody-epr16975-ab200196'>ab200196</a>)

Predicted band size: 35 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16975

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab216521 is the carrier-free version of ab200196.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The asialoglycoprotein receptor 2 (ASGR2) sometimes called Ashwell receptor subunit 2 is a transmembrane protein with a mass of approximately 46 kDa. ASGR2 combines with a similar protein ASGR1 to form a functional receptor complex. It is mostly found in the liver playing an important role in endocytosis by binding galactose-terminated glycoproteins. The receptor facilitates the clearance of these glycoproteins from the bloodstream making the liver a major site for their catabolism.

Biological function summary

ASGR2 participates in regulating serum glycoprotein levels. It is part of the asialoglycoprotein receptor complex working with ASGR1 to internalize glycoproteins for lysosomal degradation. This process is important for maintaining homeostasis of serum proteins and influencing liver function. Its activity affects hepatocyte turnover and cellular response to extracellular stimuli within the liver.

Pathways

ASGR2 is involved in the endocytosis and trafficking pathways. These pathways guide the internalization and lysosomal degradation of serum glycoproteins. ASGR2's interaction with ASGR1 through these pathways highlights its role in glycoprotein metabolism. Additionally its function intersects with the complement system's mannose-binding protein affecting immune response by clearing immune complexes.

ASGR2's dysfunction correlates with liver conditions like congenital disorders of glycosylation and hepatitis B virus infection. In hepatitis B altered ASGR2 function affects virus binding and clearance. The asialoglycoprotein receptor complex including the ASGR1/ASGR2 interaction is central to understanding changes in liver pathology and therapeutic targeting of liver diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

See full target information ASGR2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 31:746-755 PubMed39704655

2024

Smoking-Associated Carcinogen-Induced Inflammation Promotes Lung Carcinogenesis via IRAK4 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ritesh K Aggarwal,Simone Sidoli,Jingli Wang,Srabani Sahu,Rahul Sanawar,Varun Gupta,Srinivas Aluri,Vineeth Sukrithan,Charan T R Vegivinti,Phaedon D Zavras,Divij Verma,Shanisha Gordon-Mitchell,Beamon Agarwal,Tanya Verma,Daniel T Starczynowski,Ulrich G Steidl,Aditi Shastri,Balazs Halmos,Lindsay M LaFave,Haiying Cheng,Amit Verma,Yiyu Zou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASGR2 antibody [EPR16975] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

Immunoprecipitation - Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (AB216521)

관련 conjugated 항체와 다양한 조성의 항체 (8)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise