AB240896

Anti-ASIC1 antibody [S271-44]

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(5 제품이 사용된 논문 )

Mouse Monoclonal ASIC1 antibody. Suitable for ICC/IF, ICC, WB and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Asic1 aa 450-550.

대체 명칭 보기

ACCN2, BNAC2, ASIC1, Acid-sensing ion channel 1, Brain sodium channel 2

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (AB240896)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (AB240896)

Immunocytochemistry/Immunofluorescence analysis of Neuroblastoma cells (SH-SY5Y) using Anti-ASIC1 antibody [S271-44] (ab240896). Fixation : 4% PFA for 15 min. Primary Antibody : ab240896 at 1 : 100 for overnight at 4°C with slow rocking. Secondary Antibody : AlexaFluor 488 at 1 : 1000 for 1 hour at RT. Counterstain : Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1 : 800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Anti-ASIC1 antibody [S271-44] (ab240896) (D) Merged

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (AB240896)

Immunocytochemical analysis of 4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ASIC1 with ab240896 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red).

A : DAPI, B : Actin, C : ASIC1, D : Composite.

Supplier Data

Western blot - Anti-ASIC1 antibody [S271-44] (AB240896)

Lane 1:

Western blot - Anti-ASIC1 antibody [S271-44] (ab240896) at 1/100 dilution

Lane 2:

Western blot - Anti-ASIC1 antibody [S271-44] (ab240896) at 1/250 dilution

Lane 3:

Western blot - Anti-ASIC1 antibody [S271-44] (ab240896) at 1/500 dilution

Lane 4:

Western blot - Anti-ASIC1 antibody [S271-44] (ab240896) at 1/1000 dilution

All lanes:

Rat brain lysate

Predicted band size: 60 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

S271-44

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Asic1 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q6NXK8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ASIC1 or acid-sensing ion channel 1 is also known as ASIC or ACCN2. It is part of a group of proton-gated cation channels. ASIC1 has a molecular mass of approximately 60 kDa. This ion channel plays a role in the detection of acidosis where it mediates proton influx across cell membranes affecting changes in membrane potential. ASIC1 is expressed in neurons of the central and peripheral nervous systems with particular abundance in the brain. Its localization to regions such as the amygdala suggests a possible role in processing emotions and pain perception.

Biological function summary

This ion channel aids in neuronal response to pH drops serving as a sensor for acidification. The ASIC1 subunit can form homotrimers or heterotrimers with other ASIC subunits which impacts the ion conductance properties. This protein's activity affects synaptic plasticity and is involved in both short-term and long-term modulation of neuronal circuits. Changes in its activity can influence neurotransmission and has implications in neurological functionality.

Pathways

ASIC1 is part of the neurophysiological signaling pathways affecting synaptic transmission. It interacts with other proteins like ASIC2 and ASIC3 being implicated in the modulation of sensory perception and pain. Involvement in pathways like the pain-transducing signaling pathway places ASIC1 in a context where it can regulate nociceptive signals. It participates in ion flux alterations that contribute to neuronal excitability and synaptic strength.

ASIC1 has connections to neurodegenerative conditions and pain syndromes. It is implicated in the pathophysiology of disorders such as ischemia where acidosis occurs during stress and tissue damage and chronic pain conditions. ASIC1 interacts with proteins such as the NMDA receptor in the context of these diseases suggesting a role in excitotoxicity. Dysfunction or altered regulation of ASIC1 may exacerbate neurodegeneration or chronic pain symptoms highlighting its potential as a therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory (PubMed : 21036899, PubMed : 32915133, PubMed : 34319232). Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents (PubMed : 21036899). Displays high selectivity for sodium ions but can also permit the permeation of other cations (PubMed : 21036899). Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear (By similarity).. Isoform Asic1a. Has high selectivity for sodium ions, but can also be permeable to other cations including calcium, lithium and potassium.. Isoform Asic1b. Produces acid activated currents with a reduced amplitude and inactivates faster (PubMed : 21036899). Has high selectivity for sodium ions but also supports a calcium-mediated current which is sustained and maintained as long as acidic conditions are present (PubMed : 21036899). Also potentially permeable to lithium and potassium (PubMed : 21036899).. Isoform 1. Has no measurable proton-gated sodium channel activity in vitro.

See full target information ASIC1

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cell and developmental biology 13:1582970 PubMed40677921

2025

Selective blockade of acid-sensing ion channel 1a can provide substantial hippocampal neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaai Li,Yu Cheng,Di Ma,Guangjian Li,Weixuan Zhao,Ting Jiang,Hongmei Meng

Neuropsychiatric disease and treatment 18:2181-2198 PubMed36187562

2022

Ketogenic Diet Alleviates Hippocampal Neurodegeneration Possibly via ASIC1a and the Mitochondria-Mediated Apoptotic Pathway in a Rat Model of Temporal Lobe Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Qiao,Zhenzhen Qu,Shuang Tian,Huifang Cao,Yange Zhang,Can Sun,Lijing Jia,Weiping Wang

Aging 13:10703-10723 PubMed33824228

2021

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyu Ding,Renjie Zhang,Huimin Li,Qiang Ji,Xiaomin Cheng,Rick Francis Thorne,Hubert Hondermarck,Xiaoying Liu,Cailiang Shen

Cell proliferation 54:e12941 PubMed33111436

2020

Acid-sensing ion channels regulate nucleus pulposus cell inflammation and pyroptosis via the NLRP3 inflammasome in intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kangcheng Zhao,Ran An,Qian Xiang,Gaocai Li,Kun Wang,Yu Song,Zhiwei Liao,Shuai Li,Wenbin Hua,Xiaobo Feng,Xinghuo Wu,Yukun Zhang,Abhirup Das,Cao Yang

The Biochemical journal 477:75-97 PubMed31815277

2019

Aspirin inhibits TGFβ2-induced epithelial to mesenchymal transition of lens epithelial cells: selective acetylation of K56 and K122 in histone H3.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Hyun Nam,Andrew J O Smith,Mina B Pantcheva,Ko Uoon Park,Joseph A Brzezinski,James J Galligan,Kristofer Fritz,I Michael Wormstone,Ram H Nagaraj

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASIC1 antibody [S271-44]

Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (AB240896)

Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (AB240896)

Western blot - Anti-ASIC1 antibody [S271-44] (AB240896)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise