JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB243206

Anti-ASN antibody

Be the first to review this product! 리뷰 제출

|

(2 제품이 사용된 논문 )

Rabbit Polyclonal ASN antibody. Suitable for IHC-P, WB and reacts with Arabidopsis thaliana samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Arabidopsis thaliana ASN2.

대체 명칭 보기

At5g65010, MXK3.25, ASN2, Asparagine synthetase [glutamine-hydrolyzing] 2, Glutamine-dependent asparagine synthetase 2

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASN antibody (AB243206)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASN antibody (AB243206)

Arabidopsis thin leaf section was subjected to indirect immunofluorescence analysis using the Anti-ASN antibody as the primary antibody. Goat anti-rabbit IgG labelled with Alexa 405 was used as a secondary antibody. Blue : ASN protein probed with anti-ASN antibody Red : Autofluorescence

Western blot - Anti-ASN antibody (AB243206)
  • WB

Supplier Data

Western blot - Anti-ASN antibody (AB243206)

All lanes:

Western blot - Anti-ASN antibody (ab243206) at 1/1000 dilution

All lanes:

Arabidopsis leaf extract at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 65 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Arabidopsis thaliana

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Arabidopsis thaliana ASN2.

Q9LV77

Specificity

This antibody recognizes plant ASN2 and ASN1 isoproteins.

style
left-column

보완 제품

Biochemicals

AB120726

Resveratrol, Antioxidant polyphenol

Immunocytochemistry/ Immunofluorescence - Resveratrol, Antioxidant polyphenol (AB120726)

  • 0
  • 0
Assay Kits

AB211091

MTT Assay Kit (Cell Proliferation)

Functional Studies - MTT Assay Kit (Cell Proliferation) (AB211091)

  • 5
  • 2
Assay Kits

AB197010

MTS Assay Kit (Cell Proliferation) (Colorimetric)

Biochemical assay - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

  • 0
  • 0
Assay Kits

AB139418

Propidium Iodide Flow Cytometry Kit

Flow Cytometry - Propidium Iodide Flow Cytometry Kit (AB139418)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Arabidopsis thaliana": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
보관 버퍼
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ASN also known as Asparagine Synthetase is an enzyme that plays an important role in the biosynthesis of the amino acid asparagine. It catalyzes the conversion of aspartate and glutamine to asparagine utilizing ATP. The protein has a molecular mass of approximately 65 kDa. ASN is expressed in many tissues throughout the body including the liver and pancreas where it performs its enzymatic functions.
Biological function summary

ASN contributes to cellular nitrogen metabolism and protein homeostasis by assisting cells in maintaining an adequate supply of asparagine. It operates mainly in the cytoplasm and can be part of a multiprotein complex that includes other enzymes involved in amino acid metabolism. As the body requires nitrogen balance for vital processes the role of ASN is important in ensuring a continuous supply of necessary amino acids for protein synthesis and cellular growth.

Pathways

ASN is involved in the asparagine biosynthesis pathway and is also linked with the glutamine metabolism pathway. These pathways maintain cellular balance of amino acids and are interconnected with various metabolic processes. Notably ASN interacts with proteins such as glutaminase which helps convert glutamine to glutamate a precursor involved in the same pathway of amino acid synthesis.

ASN has associations with certain types of leukemia and neuroblastoma where increased ASN expression can affect tumor cell survival. The enzyme also connects to other proteins like glutaminase which influences glutamine metabolism within cancerous cells. Overexpression of ASN in these conditions can lead to adaptations that support the growth and survival of tumor cells indicating its importance in cancer metabolism research.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.
See full target information ASN2
style
left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Plant journal : for cell and molecular biology 91:371-393 PubMed28390103

2017

ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds.

Applications

WB, IHC-P

Species

Arabidopsis thaliana, Arabidopsis thaliana

Laure Gaufichon,Anne Marmagne,Katia Belcram,Tadakatsu Yoneyama,Yukiko Sakakibara,Toshiharu Hase,Olivier Grandjean,Gilles Clément,Sylvie Citerne,Stéphanie Boutet-Mercey,Céline Masclaux-Daubresse,Fabien Chardon,Fabienne Soulay,Xiaole Xu,Marion Trassaert,Maryam Shakiebaei,Amina Najihi,Akira Suzuki

Plant, cell & environment 36:328-42 PubMed22789031

2012

Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth.

Applications

WB, IHC-P

Species

Arabidopsis thaliana, Arabidopsis thaliana

Laure Gaufichon,Céline Masclaux-Daubresse,Guillaume Tcherkez,Michèle Reisdorf-Cren,Yukiko Sakakibara,Toshiharu Hase,Gilles Clément,Jean-Christophe Avice,Olivier Grandjean,Anne Marmagne,Stéphanie Boutet-Mercey,Marianne Azzopardi,Fabienne Soulay,Akira Suzuki
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com