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AB317374

Anti-Asparagine synthetase antibody [S05-9E5]

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Rabbit Recombinant Monoclonal Asparagine synthetase antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ASNS.

대체 명칭 보기

TS11, ASNS, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase Antibody [S05-9E5] (AB317374)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase Antibody [S05-9E5] (AB317374)

Immunohistochemistry of Asparagine synthetase in paraffin-embedded human tonsil using ab317374 at dilution 1/100.

Western blot - Anti-Asparagine synthetase antibody [S05-9E5] (AB317374)
  • WB

Lab

Western blot - Anti-Asparagine synthetase antibody [S05-9E5] (AB317374)

Western blot : Anti-Asparagine synthetase Antibody [S05-9E5] ab317374 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 64 kDa in Wild-type A549 cell lysates with no signal observed at this size in ASNS knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Asparagine synthetase antibody [S05-9E5] (ab317374) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

Western blot - Human ASNS knockout A549 cell line (<a href='/ko/products/cell-lines/human-asns-knockout-a549-cell-line-ab300853'>ab300853</a>) at 20 µg

Lane 3:

Jurkat cell lysates at 20 µg

Lane 4:

HeLa cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Western blot - Anti-Asparagine synthetase Antibody [S05-9E5] (AB317374)
  • WB

Supplier Data

Western blot - Anti-Asparagine synthetase Antibody [S05-9E5] (AB317374)

All lanes:

Western blot - Anti-Asparagine synthetase Antibody [S05-9E5] (ab317374) at 1/1000 dilution

Lane 1:

K562 cell lysate

Lane 2:

HeLa cell lysate

Predicted band size: 64 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

S05-9E5

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ASNS.

P08243

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보완 제품

Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB32157

Anti-EIF2S1 (phospho S51) antibody [E90]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

  • 4
  • 27
Primary Antibodies

AB216839

Anti-ATF-4 antibody

Western blot - Anti-ATF-4 antibody (AB216839)

  • 0
  • 0
Assay Kits

AB273333

Asparagine Assay Kit (Fluorometric)

Functional Studies - Asparagine Assay Kit (Fluorometric) (AB273333)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Asparagine synthetase (ASNS) is an enzyme that catalyzes the conversion of aspartate and glutamine to asparagine and glutamate. This enzyme is also known by the alternate name ASPG. Asparagine synthetase weighs approximately 64 kDa and it is found in many tissues including the liver and pancreas. The presence of ASNS is important for the synthesis of asparagine an amino acid necessary for protein and nucleotide synthesis.
Biological function summary

ASNS drives the synthesis of asparagine through a mechanism involving the ATP-dependent conversion of substrates. It does not function as part of a larger enzyme complex but operates independently to fulfill its role. In cells asparagine produced serves as a critical building block supporting protein biosynthesis and cell proliferation particularly in rapidly dividing cells such as cancer cells.

Pathways

Asparagine synthetase plays a central role in the asparagine biosynthetic pathway. This enzyme is key for maintaining cellular amino acid homeostasis and is linked to the mTOR pathway which regulates cell growth and metabolism. Additionally ASNS interacts with proteins like glutaminase which supplies one of its essential substrates glutamine.

ASNS has a significant correlation with acute lymphoblastic leukemia (ALL) where asparagine levels affect cancer cell survival. Targeting ASNS can influence chemotherapeutic strategies given its role in providing asparagine to leukemic cells. Additionally ASNS deficiency can lead to severe neurological conditions further highlighting its importance in human health. Its interaction with glutaminase is particularly relevant as both are involved in pathways targeted in ALL treatments.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information ASNS
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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