AB239487

Anti-Aspartate Aminotransferase antibody [C1]

KO Validated
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(6 제품이 사용된 논문 )

Mouse Monoclonal Aspartate Aminotransferase antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Pig, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Aspartate aminotransferase, cytoplasmic.

대체 명칭 보기

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Paraffin-embedded human kidney tissue stained for Aspartate Aminotransferase using ab239487 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Lab

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Lanes 1 - 4 : Merged signal (red and green). Green - ab239487 observed at 46 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

ab239487 was shown to react with Aspartate Aminotransferase in wild-type HAP1 cells in western blot. Loss of signal was observed when GOT1 knockout sample was used. Wild-type and GOT1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab239487 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 0.5 µg/mL

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

GOT1 knockout HAP1 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Raw264.7 cell lysate at 20 µg

Predicted band size: 46 kDa

true

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/mL

All lanes:

Rat liver lysate

Secondary

All lanes:

HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/mL

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/mL

All lanes:

Mouse liver lysate

Secondary

All lanes:

HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/mL

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 2 µg/mL

Lane 1:

Pig brain lysate

Lane 2:

Pig heart lysate

Secondary

All lanes:

HRP-linked Rabbit anti-Mouse IgG at 1/5000 dilution

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/mL

All lanes:

Rat heart lysate

Secondary

All lanes:

HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/mL

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 5 µg/mL

All lanes:

Recombinant human aspartate aminotransferase protein

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/mL

All lanes:

Human liver lysate

Secondary

All lanes:

HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/mL

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/mL

All lanes:

Rat brain lysate

Secondary

All lanes:

HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/mL

Predicted band size: 46 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Aspartate aminotransferase, cytoplasmic.

P17174

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.

Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).

See full target information Aspartate aminotransferase, cytoplasmic

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of clinical investigation 135: PubMed39808494

2025

Impaired hydrogen sulfide biosynthesis underlies eccentric contraction-induced force loss in dystrophin-deficient skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

W Michael Southern,Erynn E Johnson,Elizabeth K Fasbender,Katherine S Fallon,Courtney L Cavazos,Dawn A Lowe,George G Rodney,James M Ervasti

Frontiers in ophthalmology 3: PubMed38725581

2023

Rod photoreceptor-specific deletion of cytosolic aspartate aminotransferase, GOT1, causes retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shubha Subramanya,Moloy T Goswami,Nicholas Miller,Eric Weh,Sraboni Chaudhury,Li Zhang,Anthony Andren,Heather Hager,Katherine M Weh,Costas A Lyssiotis,Cagri G Besirli,Thomas J Wubben

PloS one 17:e0261370 PubMed35041678

2022

Curcumin suppresses tumorigenesis by ferroptosis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuelei Cao,Yao Li,Yongbin Wang,Tao Yu,Chao Zhu,Xuezhi Zhang,Jialiang Guan

Cell cycle (Georgetown, Tex.) 21:247-260 PubMed34919012

2021

CircGOT1 promotes cell proliferation, mobility, and glycolysis-mediated cisplatin resistance via inhibiting its host gene GOT1 in esophageal squamous cell cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Zhou,Zhiyuan Guo,Xueli Lv,Xueqiang Zhang

Cell stem cell 28:1982-1999.e8 PubMed34450065

2021

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Le Qi,Misty S Martin-Sandoval,Salma Merchant,Wen Gu,Matthias Eckhardt,Thomas P Mathews,Zhiyu Zhao,Michalis Agathocleous,Sean J Morrison

Cancer immunology research 9:707-722 PubMed33875483

2021

Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xia,Zaozao Zheng,Jun-Yi Liu,Yu-Jie Chen,Jiancheng Ding,Guo-Sheng Hu,Ya-Hong Hu,Suling Liu,Wen-Xin Luo,Ning-Shao Xia,Wen Liu

제품이 사용된 논문 모두 보기

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aspartate Aminotransferase antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

Western blot - Anti-Aspartate Aminotransferase antibody [C1] (AB239487)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise