AB238106

Anti-ASPM antibody

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(7 제품이 사용된 논문 )

Rabbit Polyclonal ASPM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASPM aa 100-250.

대체 명칭 보기

MCPH5, ASPM, Abnormal spindle-like microcephaly-associated protein, Abnormal spindle protein homolog, Asp homolog

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

Paraffin-embedded human lung tissue stained for ASPM using ab238106 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

Paraffin-embedded human adrenal gland tissue stained for ASPM using ab238106 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ASPM antibody (AB238106)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASPM antibody (AB238106)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASPM (green) using ab238106 at 1/100 dilution in ICC/IF, followed by Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ASPM aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q8IZT6

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Colobus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Lar gibbon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity greater than 95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ASPM also known by its alternative name "Abnormal Spindle Microtubule Assembly" is a protein that plays an important mechanical role in cell division. It is involved in mitotic spindle formation contributing to chromosome segregation during mitosis. The ASPM protein has a mass of approximately 410 kDa. This protein is expressed notably in neural tissues and regions of high cellular proliferation such as the brain and other rapidly dividing cell populations.

Biological function summary

ASPM supports proper neural development by maintaining the orientation of the mitotic spindle during neurogenesis. It does not function alone but as part of a protein complex involved in regulating mitotic spindle assembly. It associates with other mitotic spindle-associated proteins to ensure precise cell division. Through this it influences cell fate decisions and proliferation within developing tissues.

Pathways

ASPM plays a significant role in the cell cycle particularly during the mitosis phase. It is an essential part of the mitotic spindle checkpoint pathway which ensures accurate chromosome alignment and segregation. ASPM interacts with proteins like CDK1 (Cyclin-dependent kinase 1) to modulate these processes. This involvement secures ordered progression through cell division and prevents aneuploidy by ensuring spindle apparatuses function correctly.

ASPM dysfunction is closely linked to microcephaly where individuals have a significantly reduced brain size. Genetic mutations in the ASPM gene lead to some forms of primary microcephaly. The protein's influence on neurogenesis ties closely to this condition. Additionally ASPM has associations with cancer particularly in tumors where abnormal cell proliferation occurs. Alterations in ASPM expression can coincide with other cancer-related proteins such as TP53 revealing its potential role as a biomarker in tumor development and growth.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex (PubMed : 28436967). May have a preferential role in regulating neurogenesis.

See full target information ASPM

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Open life sciences 19:20220990 PubMed39759103

2025

MFAP5 inhibits the malignant progression of endometrial cancer cells .

Applications

Unspecified application

Species

Unspecified reactive species

Guanying Liang,Zijuan Qi,Chun Du

BMC cancer 24:1151 PubMed39289658

2024

Neuroblastoma with high ASPM reveals pronounced heterogeneity and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Li,Xueyuan Lu,Fengxian Zhang,Shuo Huang,Lin Ding,Hui Wang,Suyun Chen

Translational cancer research 10:983-997 PubMed35116426

2022

Overexpression of abnormal spindle-like microcephaly-associated (ASPM) increases tumor aggressiveness and predicts poor outcome in patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Feng,Jiao Zhang,Yafang Zheng,Jianchao Liu,Tianyu Duan,Tieshuan Tian

Journal of clinical laboratory analysis 35:e23931 PubMed34398984

2021

METTL3-mediated m6A methylation of ASPM drives hepatocellular carcinoma cells growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

An Wang,Xiaofeng Chen,Dongen Li,Liang Yang,Jianshuai Jiang

Experimental and therapeutic medicine 22:1154 PubMed34504599

2021

ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wu,Chunyang Hu,Lianbao Kong

International journal of oncology 58: PubMed33786609

2021

Oncogenic role of abnormal spindle‑like microcephaly‑associated protein in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Wang,Jinghui Liang,Haixia Li,Jingyi Han,Jin Jiang,Yongmeng Li,Zitong Feng,Renchang Zhao,Hui Tian

Zhongguo fei ai za zhi = Chinese journal of lung cancer 23:29-35 PubMed31948535

2020

[Expression of ASPM in Lung Adenocarcinoma and Its Relationship with Development and Prognosis].

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Wang,Zhenyu He,Renhui Duan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASPM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

Immunocytochemistry/ Immunofluorescence - Anti-ASPM antibody (AB238106)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise