AB244431

Anti-ATAD2 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ATAD2 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATAD2 aa 1050 to C-terminus.

대체 명칭 보기

L16, PRO2000, ATAD2, ATPase family AAA domain-containing protein 2, AAA nuclear coregulator cancer-associated protein, ANCCA

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human lymph node tissue labeling ATAD2 with ab244431 at 1/500 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human bone marrow tissue labeling ATAD2 with ab244431 at 1/500 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Unknown

Immunocytochemistry - Anti-ATAD2 antibody (AB244431)

Immunocytochemistry of PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATAD2 (green) using ab244431 at 4 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human testis tissue labeling ATAD2 with ab244431 at 1/500 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ATAD2 antibody (AB244431)

All lanes:

Western blot - Anti-ATAD2 antibody (ab244431) at 0.4 µg/mL

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) plus target-specific siRNA#1, cell lysate

Lane 2:

U-2 OS plus target-specific siRNA#2, cell lysate

Lane 3:

U-2 OS plus control siRNA, cell lysate

Predicted band size: 159 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATAD2 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6PL18

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATAD2 also known as ATPase family AAA domain-containing protein 2 is a chromatin-associated AAA ATPase. This protein weighs approximately 157 kDa and functions mainly in the cell nucleus. ATAD2 is widely expressed in various tissues with high levels found in the testis indicating its possible role in testicular functions. It shows ATPase activity involving the hydrolysis of ATP which provides energy for chromatin remodeling processes.

Biological function summary

ATAD2 plays a critical role in transcription regulation by remodeling chromatin structure. As part of a complex it interacts with histone acetylation marks on chromatin therefore influencing gene expression. The protein is often involved in the regulation of genes relevant to cell cycle progression. Its interaction with transcriptional regulators highlights its importance in controlling proliferation and other cellular processes.

Pathways

The role of ATAD2 becomes essential in pathways like the cell cycle and the DNA damage response. Through these pathways it interacts with key proteins such as MYC a known regulator of cell growth and it influences the expression of genes involved in cell cycle control. It is important in maintaining genomic stability by ensuring proper chromatin dynamics during DNA replication and repair.

Aberrant expression of ATAD2 associates with cancer progression particularly in breast cancer where it is highly expressed in MCF7 cell lines. It is also implicated in hepatocellular carcinoma where it interacts with cancer-related proteins such as p53 which is a well-known tumor suppressor. These interactions suggest that ATAD2 serves as a potential target for cancer therapies focused on disrupting its interactions or functions in tumor biology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.

See full target information ATAD2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of biological sciences 20:200-217 PubMed38164169

2024

Treatment for type 2 diabetes and diabetic nephropathy by targeting Smad3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun He,Honglian Wang,Xiaocui Chen,Yu Zhong,Xiao Ru Huang,Ronald Cw Ma,Cheng Wang,Hui-Yao Lan

American journal of physiology. Cell physiology 319:C93-C104 PubMed31747529

2019

Downregulation of AAA-domain-containing protein 2 restrains cancer stem cell properties in esophageal squamous cell carcinoma via blockade of the Hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Li,Yang Yu,Baoming Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATAD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunocytochemistry - Anti-ATAD2 antibody (AB244431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Western blot - Anti-ATAD2 antibody (AB244431)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise